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RabMAb

Anti-CD162 抗体 [EPR1967Y] (ab68143)

製品の概要

  • 製品名
    Anti-CD162 antibody [EPR1967Y]
    CD162 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1967Y] to CD162
  • アプリケーション
    適用あり: WB, IP, ICC, IHC-Gmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the extracellular region of CD162

  • ポジティブ・コントロール
    • Jurkat cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab68143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 43 kDa).
IP 1/30.
ICC 1/100 - 1/250.
IHC-G Use at an assay dependent concentration. PubMed: 23635004
  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能
      A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
      (Microbial infection) Acts as a receptor for enterovirus 71.
    • 組織特異性
      Expressed on neutrophils, monocytes and most lymphocytes.
    • 翻訳後修飾
      Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
      Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 162 antibody
      • CD162 antibody
      • CD162 antigen antibody
      • CLA antibody
      • Cutaneous lymphocyte associated associated antigen antibody
      • P selectin glycoprotein ligand 1 antibody
      • P selectin glycoprotein ligand 1 precursor antibody
      • P-selectin glycoprotein ligand 1 antibody
      • PSGL 1 antibody
      • PSGL-1 antibody
      • PSGL1 antibody
      • Selectin P ligand antibody
      • SELPL_HUMAN antibody
      • SELPLG antibody
      see all

    画像

    • ab681432(Red) staining CD162 in Human bronchial tissue sections by Immunohistochemistry (Glycolmethacrylate sections). Tissue was embedded in Glycolmethacrylate and floated onto 0.2% ammonia solution prior to adherence to slides.Samples were incubated with primary antibody (1/200) for 1 hour. Samples were also stained with a neutrophil elastase antibody( Brown). Sections were counterstained with haematoxylin.

      Key: E + x200 magnification and F = x500 magnification. * = Double stained cells.

       

    • All lanes : Anti-CD162 antibody [EPR1967Y] (ab68143) at 1/500 dilution

      Lane 1 : Jurkat cell lysate at 10 µg
      Lane 2 : Recombinant CD162 protein at 5ng

      Secondary
      HRP conjugated Goat anti-rabit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Liu ZJ  et al. SDF-1a-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia. Sci Rep 6:34416 (2016). Read more (PubMed: 27713493) »
    • Blidberg K  et al. Adhesion molecules in subjects with COPD and healthy non-smokers: a cross sectional parallel group study. Respir Res 14:47 (2013). IHC-G ; Human . Read more (PubMed: 23635004) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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