Anti-CD161 抗体 [PK136] (ab22515)

製品の概要

  • 製品名Anti-CD161 antibody [PK136]
    CD161 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PK136] to CD161
  • 特異性ab22515 recognises CD161c. ab22515 may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by ab22515 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. ab22515 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes.
  • アプリケーション適用あり: Flow Cyt, IP, Functional Studiesmore details
  • 種交差性
    交差種: Mouse
    非交差種: Rat, Human
  • 免疫原

    Tissue/ cell preparation (Mouse): Spleen and bone marrow cells from CE mice

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名PK136
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IP
Functional Studies
  • 追加情報Flow Cyt: Use 10µl for 106 cells, or 5 - 10 µg/ml.
    FuncS: Complement dependent lysis: Use at a concentration of 1 - 10 µg/ml.
    IP: Use at an assay dependent dilution.
    This antibody may be suitable for in vivo depletion of NK cells.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
    • 組織特異性Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
    • 配列類似性Contains 1 C-type lectin domain.
    • 翻訳後修飾N-glycosylated. Contains sialic acid residues.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-type lectin domain family 5 member B antibody
      • CD161 antibody
      • CLEC5B antibody
      • HNKR-P1a antibody
      • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
      • Killer cell lectin-like receptor subfamily B member 1 antibody
      • KLRB1 antibody
      • KLRB1_HUMAN antibody
      • Natural killer cell surface protein P1A antibody
      • NKR antibody
      • NKR P1 antibody
      • NKR-P1A antibody
      • NKRP1 antibody
      • NKRP1A antibody
      see all

    Anti-CD161 antibody [PK136] (ab22515) 使用論文

    ab22515 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry. I have been in touch with the source of this antibody and I can tell you that whilst batches are routinely tested by flow cytometry the application of in vivo depletion was added as a result of the clone being shown to ex...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"