製品の概要

  • 製品名Anti-CD161 antibody [B199.2]
    CD161 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B199.2] to CD161
  • 特異性ab23624 recognises CD161 cell surface antigen, expressed by almost all NK cells and with a small subset of CD3 positive T cells.
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Activated human NK cells.

  • エピトープClone B199.2 recognises a similar epitope to the DX9 monoclonal antibody.
  • 特記事項Myeloma cell line: P2X63.Ag8.653

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名B199.2
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
    • 組織特異性Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
    • 配列類似性Contains 1 C-type lectin domain.
    • 翻訳後修飾N-glycosylated. Contains sialic acid residues.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-type lectin domain family 5 member B antibody
      • CD161 antibody
      • CLEC5B antibody
      • HNKR-P1a antibody
      • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
      • Killer cell lectin-like receptor subfamily B member 1 antibody
      • KLRB1 antibody
      • KLRB1_HUMAN antibody
      • Natural killer cell surface protein P1A antibody
      • NKR antibody
      • NKR P1 antibody
      • NKR-P1A antibody
      • NKRP1 antibody
      • NKRP1A antibody
      see all

    Anti-CD161 antibody [B199.2] (ab23624) 使用論文

    This product has been referenced in:
    • Barnig C  et al. Lipoxin a4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med 5:174ra26 (2013). IHC-P ; Human . Read more (PubMed: 23447017) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil)
    Specification Tonsil
    Fixative Paraformaldehyde
    Antigen retrieval step Other
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10µg/mL · Temperature: 21°C
    Username

    Dr. James Jupp

    Verified customer

    投稿 Jan 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"