The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Flow cytometry data on peripheral blood (healthy individual) stained with the ab117271 at 20µl/106 cells. The reagent allows to identify the NK cell subset CD16+ with low SSC, and DC cells CD16+. Red histogram represents Granulocyte cells, blue histogram represents Dendritic and Monocyte cells and yellow histogram represents lymphocytic cells and NK cells.