Anti-CD151 抗体 [B-E31] - BSA and Azide free (ab27406)
Key features and details
- Mouse monoclonal [B-E31] to CD151 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD151 antibody [B-E31] - BSA and Azide free
CD151 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-E31] to CD151 - BSA and Azide free -
由来種
Mouse -
特異性
Recognises the antigen PETA-3, a 32 kDa protein.
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アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to CD151. Eahy 926 cell line.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free. -
ポリ/モノ
モノクローナル -
クローン名
B-E31 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27406の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Essential for the proper assembly of the glomerular and tubular basement membranes in kidney. -
組織特異性
Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. -
関連疾患
Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 977 Human
- Omim: 602243 Human
- SwissProt: P48509 Human
- Unigene: 654379 Human
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別名
- CD151 antibody
- CD151 antigen antibody
- CD151 molecule (Raph blood group) antibody
see all
画像
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A typical staining pattern with the Ab27406 monoclonal antibody of Eahy 926 (hybridoma line derived from human endothelium and A549/8 cells) cell line.
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ICC/IF image of ab27406 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27406, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab27406 は論文での使用が確認できていません。