製品の概要
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製品名Anti-CD151 antibody [11G5a]
CD151 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [11G5a] to CD151
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由来種Mouse
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特異性ab33315 recognises the human CD151 cell surface antigen, also known as PETA-3.
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アプリケーション適用あり: IHC-P, IP, WB, Flow Cyt, ICC/IF, ELISAmore details
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種交差性交差種: Human
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免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ･コントロール
- Tonsil/spleen. WB: HT1080 whole cell lysate. ICC/IF: Human fibrosarcoma cells.
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーpH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノモノクローナル
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クローン名11G5a
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ミエローマx63-Ag8.653
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アイソタイプIgG1
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab33315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. | |
| Flow Cyt | 1/50 - 1/100. 1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. |
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| ICC/IF | Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr. | |
| ELISA | Use at an assay dependent concentration. |
ターゲット情報
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機能Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
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組織特異性Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
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関連疾患Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
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配列類似性Belongs to the tetraspanin (TM4SF) family.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 977 Human
- Omim: 602243 Human
- SwissProt: P48509 Human
- Unigene: 654379 Human
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別名
- CD151 antibody
- CD151 antigen antibody
- CD151 molecule (Raph blood group) antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - CD151 antibody [11G5a] (ab33315)](http://a.abcam.com/ps/products/33/ab33315/Images/ab33315_review_12777.jpg)
Immunocytochemistry/ Immunofluorescence - CD151 antibody [11G5a] (ab33315)This image is courtesy of an anonymous Abreviewab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.
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![Flow Cytometry - CD151 antibody [11G5a] (ab33315)](http://a.abcam.com/ps/products/33/ab33315/Images/CD151-Primary-antibodies-ab33315-5.jpg)
Flow Cytometry - CD151 antibody [11G5a] (ab33315) -
![Western blot - Anti-CD151 antibody [11G5a] (ab33315)](http://a.abcam.com/ps/products/33/ab33315/Images/ab33315-296676-33315wb.jpg)
Western blot - Anti-CD151 antibody [11G5a] (ab33315)Image courtesy of an anonymous AbReviewAnti-CD151 antibody [11G5a] (ab33315) at 1/1000 dilution + HT1080 (human fibrosarcoma cell line) whole cell lysate
Secondary
Sheep anti-mouse IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [11G5a] (ab33315)](http://a.abcam.com/ps/products/33/ab33315/Images/CD151-Primary-antibodies-ab33315-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [11G5a] (ab33315)ICC/IF image of ab33315 stained HT 1080 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33315, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96789, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Palmer TD et al. Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering and is a clinical indicator of prostate cancer progression. Cancer Res 74:173-87 (2014). Read more (PubMed: 24220242) »
- Matsumoto N et al. Role of CD151 expression in gallbladder carcinoma. Surgery 156:1212-7 (2014). IHC-P ; Human . Read more (PubMed: 24890568) »
レビューと Q&A
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