Customer reviews and FAQs

製品の概要

  • 製品名Anti-CD151 antibody [11G5a]
    CD151 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [11G5a] to CD151
  • 特異性ab33315 recognises the human CD151 cell surface antigen, also known as PETA-3.
  • アプリケーション適用あり: IHC-P, IHC-Fr, IP, WB, Flow Cyt, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ･コントロール
    • Tonsil/spleen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
Flow Cyt 1/50 - 1/100.

1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
  • 組織特異性Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
  • 関連疾患Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
  • 配列類似性Belongs to the tetraspanin (TM4SF) family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CD151 antibody
      • CD151 antigen antibody
      • CD151 molecule (Raph blood group) antibody
      • CD151 molecule antibody
      • CD151_HUMAN antibody
      • GP27 antibody
      • hemidesmosomal tetraspanin CD151 antibody
      • Membrane glycoprotein SFA-1 antibody
      • MER2 antibody
      • PETA-3 antibody
      • PETA3 antibody
      • Platelet endothelial tetraspan antigen 3 antibody
      • platelet surface glycoprotein gp27 antibody
      • Platelet-endothelial cell tetraspan antigen 3 antibody
      • Platelet-endothelial tetraspan antigen 3 antibody
      • RAPH antibody
      • Red blood cell antigen MER2 antibody
      • SFA 1 antibody
      • SFA1 antibody
      • Tetraspanin-24 antibody
      • Tspan-24 antibody
      • TSPAN24 antibody
      see all

    Anti-CD151 antibody [11G5a] 画像

    • Immunocytochemistry/ Immunofluorescence - CD151 antibody [11G5a] (ab33315)
      Immunocytochemistry/ Immunofluorescence - CD151 antibody [11G5a] (ab33315)This image is courtesy of an anonymous Abreview

      ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.  

      See Abreview

    • Flow Cytometry - CD151 antibody [11G5a] (ab33315)
      Flow Cytometry - CD151 antibody [11G5a] (ab33315)

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [11G5a] (ab33315)
      Immunocytochemistry/ Immunofluorescence - Anti-CD151 antibody [11G5a] (ab33315)
      ICC/IF image of ab33315 stained HT 1080 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33315, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96789, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-CD151 antibody [11G5a] (ab33315) 使用論文

    This product has been referenced in:
    • Palmer TD  et al. Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering and is a clinical indicator of prostate cancer progression. Cancer Res 74:173-87 (2014). Read more (PubMed: 24220242) »
    • Matsumoto N  et al. Role of CD151 expression in gallbladder carcinoma. Surgery 156:1212-7 (2014). IHC-P ; Human . Read more (PubMed: 24890568) »

    See all 5 Publications for this product

    ab33315 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab33315 の abreview を投稿する ab33315 に関する問い合わせをする

    5 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-CD151 antibody [11G5a]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HT1080)
    Loading amount 100000 cells
    Specification HT1080
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
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    Verified customer

    投稿 May 12 2012

    which antigen retrieval step was used for IHC-P?

    Thank you for contacting us. The antigen retrieval step would be heat-mediated with citrate buffer (pH6.0). Details can be found here: Heat-induced epitope retrieval (HIER) Heat-mediated antigen retrieval may be used in ma...

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    Flow Cytometry abreview for Anti-CD151 antibody [11G5a]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (Fibrosarcoma)
    Specification Fibrosarcoma
    Preparation Cell harvesting/tissue preparation method: Cells lifted off plastic in PBS/EDTA
    Sample buffer: PBS/EDTA
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy viable cells
    Read More
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    Verified customer

    投稿 Oct 06 2010

    Flow Cytometry abreview for Anti-CD151 antibody [11G5a]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (Fibrosarcoma HT1080)
    Specification Fibrosarcoma HT1080
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy no gate
    Read More
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    Abcam user community

    Verified customer

    投稿 Apr 07 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD151 antibody [11G5a]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (fibrosarcoma)
    Specification fibrosarcoma
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Image FX signal enhancer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
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    Verified customer

    投稿 Dec 02 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"