The details of the immunogen for this antibody are not available.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Our Abpromise guarantee covers the use of ab33315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. | |
Flow Cyt | 1/50 - 1/100. 1/50 - 1/100. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. |
|
ICC/IF | Use at an assay dependent concentration. Use at a concentration of 10 µg/ml for 1 hr. | |
ELISA | Use at an assay dependent concentration. |
ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"