Anti-CD15 抗体 [28] (FITC) (ab82537)

製品の概要

  • 製品名Anti-CD15 antibody [28] (FITC)
    CD15 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [28] to CD15 (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: IP, IHC-Fr, Flow Cyt, Inhibition Assaymore details
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. Inhibition of neutrophil phagocytosis and bactericial activity.

ターゲット情報

  • 関連性CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • 細胞内局在Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • 参照データベース
  • 別名
    • 3-FAL antibody
    • Alpha-3-fucosyltransferase antibody
    • ELAM 1 ligand antibody
    • ELAM ligand fucosyltransferase antibody
    • ELAM-1 ligand fucosyltransferase antibody
    • ELFT antibody
    • FCT3A antibody
    • FUC-TIV antibody
    • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) antibody
    • Fucosyltransferase antibody
    • fucosyltransferase IV antibody
    • fucT-IV antibody
    • FUTIV antibody
    • galactoside 3-L-fucosyltransferase antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Anti-CD15 antibody [28] (FITC) 画像

  • Overlay histogram showing HL60 cells stained with ab82537 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82537, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgGM (FITC) (2µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab82537 staining CD15 in Human primary glioblastoma cell line by Flow Cytometry. Cells harvested, fixed with 70% ice-cold ethanol and permeabilized with Triton X-100 0.1%. The sample was incubated with the antibody (1/10) for 60 minutes.
    Gating Strategy: Cells incubated with a mouse IgG-FITC.

    See Abreview

Anti-CD15 antibody [28] (FITC) (ab82537) 使用論文

ab82537 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Human primary glioblastoma cell line)
Specification Human primary glioblastoma cell line
Fixation 70% ice-cold ethanol
Permeabilization Yes - 0.1% Triton X-100
Gating Strategy Cells incubated with a mouse IgG-FITC
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投稿 Aug 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"