The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant mouse EMMPRIN is approximately 0.2 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for mouse EMMPRIN is approximately 1 ng/lane under non-reducing and reducing conditions. Detects a band of approximately 58 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
組織特異性Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.