製品の概要

  • 製品名Anti-CD14 antibody [Tuk4]
    CD14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Tuk4] to CD14
  • 特異性Ab27545 recognises CD14 surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes.
  • アプリケーション適用あり: WB, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Sheep, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Mink (Mustela), Cynomolgus Monkey
  • 特記事項

    Clone Tuk4 has been shown to block SDF induced chemotaxis of U937 cells in a dose dependent manner. This antibody could therefore be recommended for this purpose.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

Use under reducing conditions.

ELISA Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200.

Use 5µl of the suggested working dilution to label 106 cells or 100µl whole blood.





ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 組織特異性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 配列類似性Contains 11 LRR (leucine-rich) repeats.
  • 翻訳後修飾N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [Tuk4] (ab27545) 使用論文

This product has been referenced in:
  • Slobedman B  et al. Latent cytomegalovirus down-regulates major histocompatibility complex class II expression on myeloid progenitors. Blood 100:2867-73 (2002). Read more (PubMed: 12351397) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (normal dog spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid / Tris EDTA
Permeabilization No
Specification normal dog spleen
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
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投稿 May 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"