製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to CD14
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    C-KRVDADADPRQYAD

    , corresponding to Internal sequence amino acids 71-84 of Human CD14

  • ポジティブ・コントロール
    • Human Lymph Node lysate and tonsil lysates. Human spleen tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 組織特異性
    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 配列類似性
    Contains 11 LRR (leucine-rich) repeats.
  • 翻訳後修飾
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody 画像

  • Anti-CD14 antibody (ab45870) at 1 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer).

    Predicted band size : 40 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab45870 at 4µg/ml staining CD14 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Image shows strong clustered staining in some splenocytes.

Anti-CD14 antibody (ab45870) 使用論文

This product has been referenced in:
  • Herter S  et al. A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents. Cancer Immunol Immunother 66:129-140 (2017). IHC-P ; Human . Read more (PubMed: 27858101) »
  • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). Read more (PubMed: 25845433) »

See all 6 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with product ab45870. To check the status of the order please contact our Cust...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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