Anti-CD14 抗体 [61D3] (PE/Cy5®) (ab25395)


  • 製品名Anti-CD14 antibody [61D3] (PE/Cy5®)
    CD14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [61D3] to CD14 (PE/Cy5®)
  • 標識PE/Cy5®. Ex: 496nm, Em: 670nm
  • 特異性Human CD14
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.



Our Abpromise guarantee covers the use of ab25395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • 機能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 組織特異性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 配列類似性Contains 11 LRR (leucine-rich) repeats.
  • 翻訳後修飾N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [61D3] (PE/Cy5®) 画像

  • Flow cytometry analysis of lymphocytes, staining CD14 with ab25395.

    Cells were isolated from Ficoll-Paque density gradient in 2% human serum, 0.5 mM EDTA in PBS. The sample was incubated with the primary antibody (1/100 in 2% human serum, 0.5 mM EDTA in PBS) for 20 minutes at 4°C.

    Gating Strategy:Monocytes

    See Abreview

Anti-CD14 antibody [61D3] (PE/Cy5®) (ab25395) 使用論文

This product has been referenced in:
  • Demers KR  et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog 12:e1005805 (2016). Read more (PubMed: 27486665) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. The CD14 antibody is already conjugated to PE/CY5, so I wouldn't recommend trying to use the kit to conjugate it to something else. We do have an unconjugated version of the same antibody with catalog ab25625.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (peripheral blood cells)
Specification peripheral blood cells
Preparation Cell harvesting/tissue preparation method: Lymphocytes isolated from Ficoll-Paque density gradient.
Sample buffer: 2% human serum, 0.5 mM EDTA in PBS
Permeabilization No
Gating Strategy monocytes

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投稿 Sep 04 2012