製品の概要

  • 製品名Anti-CD14 antibody [47-3D6] (PerCP)
    CD14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [47-3D6] to CD14 (PerCP)
  • 標識PerCP. Ex: 482nm, Em: 675nm
  • 特異性ab91146 reacts with human monocytes and macrophages; weak reactions may occur with neutrophils.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human lung angiotensin converting enzyme.

  • ポジティブ・コントロール
    • Human Normal peripheral blood

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 20µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 組織特異性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 配列類似性Contains 11 LRR (leucine-rich) repeats.
  • 翻訳後修飾N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [47-3D6] (PerCP) 画像

  • ab91146 staining CD14 in the Human blood cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the undiluted primary antibody for 30 minutes at 4°C.

    See Abreview

  • ab91146, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.

Anti-CD14 antibody [47-3D6] (PerCP) (ab91146) 使用論文

This product has been referenced in:
  • Hünniger K  et al. A virtual infection model quantifies innate effector mechanisms and candida albicans immune escape in human blood. PLoS Comput Biol 10:e1003479 (2014). Flow Cyt ; Human . Read more (PubMed: 24586131) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation Formaldehyde
Permeabilization No
Sample Human Cell (Whole blood)
Specification Whole blood
Gating Strategy Lymphocytes, Granulocytes and Monocytes
Preparation Cell harvesting/tissue preparation method: Whole blood collected in ACD
Sample buffer: None
Username

Abcam user community

Verified customer

投稿 Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient
Sample buffer: PBS
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

投稿 Apr 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"