製品の概要

  • 製品名Anti-CD14 antibody [2Q1233]
    CD14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2Q1233] to CD14
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human peripheral blood mononuclear cells.

  • ポジティブ・コントロール
    • Peripheral blood monocytes.
  • 特記事項Though we have received a positive Abreview for this antibody in IHC-P, we have also received many negative reports in IHC-P and so we can no longer guarantee this antibody in this application. We appreciate any further feedback from users in this regard.


    ab63319 reacts with peripheral blood monocytes and weakly with granulocytes. It is negative to platelets, erythrocytes and lymphocytes. It is sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). It is negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/25. Dilute with isotonic buffer. Use 50ul per 1x106 peripheral blood mononuclear cells (PBMC) in 100ul buffer.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/25.
IHC-Fr 1/25. We recommend acetone fixation.

ターゲット情報

  • 機能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 組織特異性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 配列類似性Contains 11 LRR (leucine-rich) repeats.
  • 翻訳後修飾N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [2Q1233] 画像

  • ab63319 staining CD14 in human tonsil tissue sections by IHC-Fr (formaldehyde-fixed frozen sections). Tissue samples were fixed with formaldehyde; antigen retrival was by heat mediation in EDTA (pH8) for 20 minutes at 100°C. The sample was incubated with primary antibody (1/300) at 25°C for 20 minutes. An undiluted HRP-conjugated Goat polyclonal to mouse/rabbit IgG was used as secondary antibody.

    See Abreview

Anti-CD14 antibody [2Q1233] (ab63319) 使用論文

This product has been referenced in:
  • Erikson E  et al. In vivo expression profile of the antiviral restriction factor and tumor-targeting antigen CD317/BST-2/HM1.24/tetherin in humans. Proc Natl Acad Sci U S A 108:13688-93 (2011). Flow Cyt ; Human . Read more (PubMed: 21808013) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral macrophages)
Specification Peripheral macrophages
Preparation Cell harvesting/tissue preparation method: Ficoll gradient separation/adhesion
Sample buffer: PBS
Permeabilization No
Gating Strategy FL1
Username

Abcam user community

Verified customer

投稿 Mar 18 2013

According to our records, ab39256, ab63319, ab77258, ab86673, ab103674 was proving difficult to use in [APPL] and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more detail...

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Thank you for your email.

We will look forward to receive the images. On the optimization note we strongly recommend to include the blocking step in protocol; please try 5%BSA or 5-10% normal serum for 1-2 hours at room temperature with specie...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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Thank you for your email.

I am sorry this product did not perform in IHC-P as originally stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx wi...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 minutes
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Dec 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"