Anti-CD134 / OX40L receptor 抗体 [EPR17Y] (ab76000)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD134 / OX40L receptor antibody [EPR17Y]
CD134 / OX40L receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CD134/ OX40L receptor aa 1-250. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human lymph node, spleen and tonsil tissue lysates and PMBC cell lysate treated with TPA.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.19% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Receptor for TNFSF4/OX40L/GP34. -
配列類似性
Contains 4 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7293 Human
- Omim: 600315 Human
- SwissProt: P43489 Human
- Unigene: 129780 Human
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別名
- ACT 35 antibody
- ACT35 antibody
- ACT35 antigen antibody
see all
画像
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Flow cytometry analysis of Human PBMC treated with 1©:g/ml PHA for 3 days cells labelling CD134 / OX40L receptor with ab76000 at 1/1200 dilution (1.05 μg/ml) (Red). Dylight488 (Ab98462) was used as the secondary antibody at 1/2000 dilution.
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All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution
Lane 1 : Human lymph node tissue lysate
Lane 2 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution + Human tonsil tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution (unpurified)
Lane 1 : PBMC cell lysates, untreated
Lane 2 : PBMC cell lysates, treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Note: the actual molecular weight is larger than the predicted due to glycoslyation.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76000 は 2 報の論文で使用されています。
- Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . PubMed: 27882144
- Byun M et al. Inherited human OX40 deficiency underlying classic Kaposi sarcoma of childhood. J Exp Med 210:1743-59 (2013). WB, Flow Cyt ; Human . PubMed: 23897980