製品の概要

  • 製品名Anti-CD13 antibody [WM15]
    CD13 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [WM15] to CD13
  • アプリケーション適用あり: Functional Studies, WB, ELISA, Flow Cyt, IHC-Fr, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human AML cells

  • エピトープThe antibody recognizes an extracellular epitope.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration. The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.
WB Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IP Use at 10 µg/mg of lysate.
ICC/IF Use a concentration of 10 µg/ml. See Abreview.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
  • 組織特異性Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
  • 配列類似性Belongs to the peptidase M1 family.
  • ドメインAmino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
  • 翻訳後修飾Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.
  • 細胞内局在Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alanyl (membrane) aminopeptidase antibody
    • Alanyl aminopeptidase antibody
    • Aminopeptidase M antibody
    • Aminopeptidase N antibody
    • AMPN_HUMAN antibody
    • ANPEP antibody
    • AP M antibody
    • AP N antibody
    • AP-M antibody
    • AP-N antibody
    • APN antibody
    • CD 13 antibody
    • CD13 antibody
    • CD13 antigen antibody
    • gp150 antibody
    • hAPN antibody
    • LAP 1 antibody
    • LAP1 antibody
    • Microsomal aminopeptidase antibody
    • Myeloid plasma membrane glycoprotein CD13 antibody
    • p150 antibody
    • PEPN antibody
    see all

Anti-CD13 antibody [WM15] 画像

  • ab7417 staining CD13 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 20 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/400) for 18 hours at 20°C. An undiluted HRP-conjugated Horse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab7417 staining CD13 on the surface of Human peripheral blood leukocytes in Flow Cytometry.

Anti-CD13 antibody [WM15] (ab7417) 使用論文

This product has been referenced in:
  • Principe S  et al. Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine. J Proteome Res 11:2386-96 (2012). WB . Read more (PubMed: 22339264) »
  • Favaloro EJ  et al. The hepatobiliary disease marker serum alanine aminopeptidase predominantly comprises an isoform of the haematological myeloid differentiation antigen and leukaemia marker CD-13/gp150. Clin Chim Acta 220:81-90 (1993). Read more (PubMed: 7904544) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Treatment Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

投稿 Jan 10 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Sample Human Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Miss. Laura Palmer

Verified customer

投稿 Dec 17 2013

Thank you for your reply.
I cannot recommend this antibody for your purposes. Rat still shows a 77% homology and I am afraid that this could be boarder line and this antibody might cross-react.
It is always very difficult to positively proof ...

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Thank you for your inquiry.
This antibody is tested and guaranteed to work with human samples. Other species were not experimentally tested by us and we simply do not know i this antibody will cross-react.
Human CD13 show a high homology with...

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I am very pleased to hear you would like to accept our offer and test ab7417 in bovine samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To rede...

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Thank you very much for your interest in ab7417.

To our knowledge, ab7417 has not been tested in on bovine samples, but based on sequence homology (there is a 87% level of homology between human and bovine), there is a high chance that this ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Fibrosarcoma HT1080)
Specification Fibrosarcoma HT1080
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy no gate
Username

Abcam user community

Verified customer

投稿 Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Fibrosarcoma)
Specification Fibrosarcoma
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy Live cells
Username

Abcam user community

Verified customer

投稿 Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (fibrosarcoma)
Total protein in input 1000 µg
Specification fibrosarcoma
Immuno-precipitation step Protein G
Username

Miss. Helen Gillingham

Verified customer

投稿 Apr 09 2008

Thank you for your enquiry. The best choice for a lysate would be a membrane fraction lysate, not a nuclear lysate, since CD13 is a trans-membrane protein. The antigen is expressed on a variety of tissues. The best choice is ab7913, THP1 (Human acute m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"