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Our Abpromise guarantee covers the use of ab7417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at 10 µg/mg of lysate.|
|ICC/IF||Use a concentration of 10 µg/ml. See Abreview.|
|IHC-P||Use at an assay dependent concentration.|
ab7417 staining CD13 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 20 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/400) for 18 hours at 20°C. An undiluted HRP-conjugated Horse anti-mouse IgG polyclonal was used as the secondary antibody.
Lane 1: Control (no antibody used for IP)
Lane 2: Anti CD13 [WM15], ab7417
Ab7417 immunoprecipitating CD13 in Fibrosarcoma whole cell lysates. For western blotting, primary antibody used was ab7417 at 10µg/mg lysate. Sample was incubated for 2hours at 4°C. A HRP conjugated Rabbit pAb was used as the secondary antibody at 1/100 dilution.
Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).
Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"