製品の概要

  • 製品名
    Anti-CD13 antibody [EPR4058]
    CD13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4058] to CD13
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in human CD13

  • ポジティブ・コントロール
    • THP-1 cell lysate, human fetal liver lysate, human kidney tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • 組織特異性
      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • 配列類似性
      Belongs to the peptidase M1 family.
    • ドメイン
      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • 翻訳後修飾
      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • 細胞内局在
      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Anti-CD13 antibody [EPR4058] 画像



    • Predicted band size : 110 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: CD13 (KO) whole cell lysate (20 µg)
      Lane 4: Empty
      Lane 5: HeLa whole cell lysate (20 µg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE.  Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution

      Lane 1 : THP-1 cell lysate at 10 µg
      Lane 2 : human fetal liver lysate


      Performed under reducing conditions.

      Predicted band size : 110 kDa
    • ab108310, at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.
    • ab108310 showing positive staining in Normal liver tissue.

    • ab108310 showing positive staining in Hepatocellular carcinoma tissue.

    • ab108310 showing positive staining in Astrocytoma tissue.

    • ab108310 showing positive staining in Normal breast tissue.

    • ab108310 showing positive staining in Prostatic carcinoma tissue.

    • ab108310 showing positive staining in Normal tonsil tissue.

    • ab108310 showing positive staining in Normal stomach tissue.

    • ab108310 showing positive staining in Normal colon tissue.

    • ab108310 showing positive staining in Lung adenocarcinoma tissue.

    Anti-CD13 antibody [EPR4058] (ab108310) 使用論文

    This product has been referenced in:
    • Harada K  et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). Read more (PubMed: 27464948) »
    • Cui SX  et al. 13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3 tripeptide, inhibits human colonic carcinoma growth by targeting Aminopeptidase N (APN/CD13). Eur J Pharmacol 734:50-9 (2014). Read more (PubMed: 24726845) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (trochlea bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    trochlea bone
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 06 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Jejunum)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Jejunum
    Fixative
    Paraformaldehyde
    Username

    Lina Li

    Verified customer

    投稿 May 31 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (brain pericytes)
    Specification
    brain pericytes
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Mrs. Verena Ledwig

    Verified customer

    投稿 Mar 09 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Normal kidney
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Feb 24 2016

    Thank you or your inquiry. I am happy to confirm that ab108310 (Anti-CD13 antibody [EPR4058]) is tested and guaranteed for ICC (Immunocytochemistry, which includes cell smears) and IHC-P with Mouse, Rat and Human samples. This antibody has not yet been...

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    Thank you for contacting us. Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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