製品の概要
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製品名Anti-CD11c antibody [N418]
CD11c 一次抗体 製品一覧 -
製品の詳細Armenian Hamster monoclonal [N418] to CD11c
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由来種Armenian hamster
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特異性ab33483 recognizes the murine homologue of human CD11c (150/90kD) integrin family. This product is recommended for IHC-Fr only although some customers have used it successfully in IHC-P.
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アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-Fr, IPmore details
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種交差性交差種: Mouse
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免疫原
Tissue, cells or virus corresponding to Mouse CD11c.
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ポジティブ･コントロール
- IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
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特記事項
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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一次抗体 備考Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
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ポリ/モノモノクローナル
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クローン名N418
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ミエローマSp2/0
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
- Anti-CD11c antibody [N418] (PerCP/Cy5.5®) (ab111469)
- Anti-CD11c antibody [N418] - Low endotoxin, Azide free (ab185783)
- Anti-CD11c antibody [N418] (Allophycocyanin) (ab210306)
- Anti-CD11c antibody [N418] (Allophycocyanin/Cy7 ®) (ab210307)
- Anti-CD11c antibody [N418] (FITC) (ab210308)
- Anti-CD11c antibody [N418] (Phycoerythrin) (ab210309)
- Anti-CD11c antibody [N418] (PE/Cy7 ®) (ab210310)
- Anti-CD11c antibody [N418] (Biotin) (ab95765)
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab33483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| ICC/IF | Use at an assay dependent concentration. PubMed: 20808846 | |
| Flow Cyt | 1/100. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. Abcam recommends using acetone fixation. |
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| IP | Use at an assay dependent concentration. |
ターゲット情報
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機能Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
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組織特異性Predominantly expressed in monocytes and granulocytes.
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配列類似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 16411 Mouse
- SwissProt: Q9QXH4 Mouse
- Unigene: 22378 Mouse
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別名
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
画像
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![Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)](http://a.abcam.com/ps/products/33/ab33483/Images/ab33483-268188-ab33483CD3FLOW2.jpg)
Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes. -
![Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)](http://a.abcam.com/ps/products/33/ab33483/Images/ab33483-254026-ab3348350886.jpg)
Flow Cytometry - Anti-CD11c antibody [N418] (ab33483)Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD11c antibody [N418] (ab33483)](http://a.abcam.com/ps/products/33/ab33483/Images/CD11c-Primary-antibodies-ab33483-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD11c antibody [N418] (ab33483)Image courtesy of Wang J et al, PLoS Pathog. 2010 Aug 19;6(8). pii: e1001058, Fig 5.ab33483 staining CD11c in murine bronchoalveolar lavage (BAL) cells by Immunocytochemistry/ Immunofluorescence.
BAL cells were collected from PBS treated mice at day 13, 17 or 18 (17/18) and 21 post-reconstitution. Cells were stained with ab33483 specific for CD11c (green) and Pc (red). Representative images of brightfield (BF), CD11c, Pc, and merged CD11c/Pc are shown for PBS-treated mice following immune reconstitution Day 21.
プロトコール
参考文献
This product has been referenced in:
- Tan CSE et al. CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection. PLoS Pathog 13:e1006311 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28394921) »
- Meng Y et al. Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells. Int J Mol Med 39:1101-1110 (2017). Read more (PubMed: 28339016) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"