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Spleen dendritic cells (Mouse)
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Our Abpromise guarantee covers the use of ab33483 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20808846|
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.
Abcam recommends using acetone fixation.
|IP||Use at an assay dependent concentration.|
Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.