Tissue, cells or virus corresponding to Mouse CD11c.
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Our Abpromise guarantee covers the use of ab33483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use at an assay dependent concentration. PubMed: 20808846 | |
Flow Cyt | 1/100. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr | Use at an assay dependent concentration. Abcam recommends using acetone fixation. |
|
IP | Use at an assay dependent concentration. |
Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.
Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.
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