製品の概要

  • 製品名Anti-CD11c antibody [EP1347Y]
    CD11c 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1347Y] to CD11c
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IF, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD11c aa 1150 to the C-terminus (C terminal).
    Database link: P20702

  • ポジティブ・コントロール
    • THP-1 + TPA lysate. Human lymph node. Flow: human leucocytes
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CD11c antibody (HRP) [EP1347Y] (ab195534)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa).
IP 1/80.
Flow Cyt 1/100.

ターゲット情報

  • 機能Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • 組織特異性Predominantly expressed in monocytes and granulocytes.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

Anti-CD11c antibody [EP1347Y] 画像

  • Human peripheral blood lymphocytes stained with anti-CD11c antibody ab52632. Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were double stained with anti-CD3 PE-conjugated (ab27349) and purified anti-CD11c (ab52632) at 1/100 for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C.

    Acquisition of >30,000 total events were collected. Gating strategy - peripheral blood lymphocytes

  • Human peripheral blood lymphocytes stained with anti-CD11c antibody ab52632. Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were double stained with anti-CD19 APC-conjugated (ab91178) and purified anti-CD11c (ab52632) at 1/100 for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C.

    Acquisition of >30,000 total events were collected Gating strategy - peripheral blood lymphocytes

  • Anti-CD11c antibody [EP1347Y] (ab52632) at 1/1000 dilution + THP-1 + TPA lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 128 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CD11c antibody [EP1347Y] (ab52632) at 1/1000 dilution

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab52632 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab52632 showing positive staining in Normal tonsil tissue.

  • ab52632 at 1/400 dilution staining CD11c in human tonsil tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, prior to blocking in 10% BSA in FCS (20ml) + DMEM (80ml) for 30 minutes at 21°C and then incubated with ab52632 for 20 hours at 4°C. A biotin conjugated rabbit polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • ab52632 showing negative staining in Normal brain tissue.

  • ab52632 showing negative staining in Normal breast tissue.

  • ab52632 showing negative staining in Skeletal muscle tissue.

  • ab52632 showing positive staining in Normal spleen tissue.

Anti-CD11c antibody [EP1347Y] (ab52632) 使用論文

This product has been referenced in:
  • Morandi F  et al. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol 192:2634-42 (2014). Read more (PubMed: 24554774) »
  • Wehrhan F  et al. Increased malignancy of oral squamous cell carcinomas (oscc) is associated with macrophage polarization in regional lymph nodes - an immunohistochemical study. BMC Cancer 14:522 (2014). IHC-P ; Human . Read more (PubMed: 25042135) »

See all 14 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 May 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Permeabilization No
Specification Skin
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Formaldehyde
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投稿 May 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Sarcoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification Human Sarcoma
Blocking step Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Formaldehyde
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Verified customer

投稿 Nov 09 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization No
Specification Lymph node
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative Formaldehyde
Username

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Verified customer

投稿 Aug 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Rabbit Tissue sections (Tonsil)
Specification Tonsil
Permeabilization Yes - 0.2% Tween in PBS
Fixative Formaldehyde
Username

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Verified customer

投稿 Nov 25 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6.0
Sample Cynomolgus Monkey Tissue sections (Tonsil, lymph node, spleen, thymus)
Specification Tonsil, lymph node, spleen, thymus
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: Rt°C
Fixative Formaldehyde
Username

Jing Ma

Verified customer

投稿 Dec 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 16.7% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6.1
Sample Human Tissue sections (colon)
Specification colon
Permeabilization No
Fixative Formaldehyde
Username

Ms. Judith Gras

Verified customer

投稿 Sep 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 16.7% · Temperature: 22°C
Sample Human Tissue sections (colon)
Specification colon
Permeabilization Yes - Acetone
Fixative Acetone
Username

Ms. Judith Gras

Verified customer

投稿 Sep 24 2013


Yes, if the datasheet says does not react with mouse , it means this antibody will not worl on mouse samples.
We do have mouse specific antibodies for Anti-CD11c raised in hamster which works really well.
A suggested antibody that is wid...

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Kein Problem - wir haben soeben den Discount Code hinzugefügt. Ich kann Ihnen bestätigen, dass dieser jetzt aktiv ist.

Es freut mich auch zu sehen, dass der Antikörper auf gefroren Schnitten funktioniert!

Bitte zö...

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1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"