This antibody recognises the integrin alphax subunit, a 150kD glycoprotein expressed by macrophages, monocytes, granulocytes and NK cells. The antigen is also expressed by hairy cell leukaemia cells. Ligands for CD11c are ICAM 1, iC3b, and fibrinogen. The molecule has a role in mediating adherence of granulocytes and monocytes to endothelium and fibrinogen.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl of working dilution for 106 cells. Suggested working dilution is neat - 1/10.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Predominantly expressed in monocytes and granulocytes.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Rezzonico R et al. Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood97:2932-40 (2001).
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