製品の概要

  • 製品名
    Anti-CD11c antibody [3.9]
    CD11c 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3.9] to CD11c
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IP, Flow Cyt, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Rheumatoid synovial fluid cells and fibronectin purified human monocytes.

  • ポジティブ・コントロール
    • Flow Cyt: Human Leucocytes

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/150.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt 1/100.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • 組織特異性
    Predominantly expressed in monocytes and granulocytes.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメイン
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen like family member C antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

画像

  • ab11029 staining CD11c in murine placenta and uterine tissue sections by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 25°C. Samples were incubated with primary antibody (1/25 in 1% BSA in PBS) for 1 hour at 25°C. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used to detect staining.

    See Abreview

  • Human peripheral blood lymphocytes stained with ab11029. Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were stained with anti-CD11c ab11029 (right panel) at 1/100 dilution for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (left panel) was mouse monoclonal IgG1 (ab170190) used under the same conditions.

    Acquisition of >30,000 total events were collected using a 50mW Argon Blue laser (488nm) and 530/30 bandpass filter. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable cells.

  • ab11029 staining CD11c in Mouse placenta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% BSA) for 90 minutes. An undiluted HRP-conjugated  anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Xue N  et al. Chlorogenic acid inhibits glioblastoma growth through repolarizating macrophage from M2 to M1 phenotype. Sci Rep 7:39011 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28045028) »
  • Zhu Y  et al. Identification of different macrophage subpopulations with distinct activities in a mouse model of oxygen-induced retinopathy. Int J Mol Med 40:281-292 (2017). IF ; Mouse . Read more (PubMed: 28627621) »

See all 12 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Inflammed lung tissue)
Permeabilization
No
Specification
Inflammed lung tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Formaldehyde
Username

Dr. Jim Hsiao

Verified customer

投稿 May 01 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Wesley Konsavage

Verified customer

投稿 Dec 22 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (oral mucosa (tongue))
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Specification
oral mucosa (tongue)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Luciana Correa

Verified customer

投稿 Jun 18 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Tissue sections (Breast tumor)
Specification
Breast tumor
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Gajendra Katara

Verified customer

投稿 Sep 05 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Cell (Peritoneal macrophages)
Specification
Peritoneal macrophages
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 21 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
casein in PBS- DAKO ready to use sera-free protein block as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Traci Lyons

Verified customer

投稿 Nov 13 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample
Mouse Tissue sections (Uterine Decidua)
Specification
Uterine Decidua
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

投稿 Nov 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Placenta)
Specification
Placenta
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3µg/mL · Temperature: RT°C
Username

Dr. Mukesh Jaiswal

Verified customer

投稿 Sep 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Placenta and uterus)
Specification
Placenta and uterus
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

投稿 Sep 18 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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