Anti-CD11b/c 抗体 [OX42] (Phycoerythrin) (ab112239)

製品の概要

  • 製品名
    Anti-CD11b/c antibody [OX42] (Phycoerythrin)
    CD11b/c 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OX42] to CD11b/c (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性
    ab112239 recognizes most macrophages including resident peritoneal macrophages, kupffer cells, but only approximately 35% of alveolar macrophages. ab112239 also labels dendritic cells extensively, granulocytes and cells with morphology of microglia in the brain. It precipitates three polypeptides of M.W. 160, 103 and 95kDa. ab112239 inhibits complement mediated rosettes and is probably the rat equivalent of the Human receptor for iC3b called CR3.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Rat Peritoneal Macrophages

  • ポジティブ・コントロール
    • Peritoneal Macrophages from rat strains Wistar, Buffalo, Brown Norway and Fischer 344.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab112239 was purified from ascitic fluid via Protein G Chromatography
  • ポリ/モノ
    モノクローナル
  • クローン名
    OX42
  • ミエローマ
    NS0/U
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.05µg for 106 cells. ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 組織特異性
    Predominantly expressed in monocytes and granulocytes.
  • 関連疾患
    Systemic lupus erythematosus 6
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメイン
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen CD11b (p170) antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen like family member C antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11C antibody
    • Cell surface glycoprotein MAC 1 subunit alpha antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3A antibody
    • Integrin alpha M antibody
    • Integrin alpha X antibody
    • Integrin alpha-M antibody
    • Integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • Integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • Integrin, alpha X (complement component 3 receptor 4 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • ITGAX antibody
    • Leu M5, alpha subunit antibody
    • Leukocyte adhesion glycoprotein p150,95 alpha chain antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Leukocyte adhesion receptor p150,95 antibody
    • Leukocyte surface antigen p150,95, alpha subunit antibody
    • MAC 1 antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • MO1A antibody
    • Myeloid membrane antigen, alpha subunit antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • OTTHUMP00000163299 antibody
    • p150 95 integrin alpha chain antibody
    • SLEB6 antibody
    see all

画像

  • ab112239 at 0.05 µg/106 cells detecting CD11b/c in Wistar Rat Peritoneal Macrophage cells by Flow Cytometry. 97% more of the cells were stained than in the control. Rat strains Buffalo, Brown Norway and Fischer 344 were also positive in this assay.

プロトコール

参考文献

This product has been referenced in:
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »
  • Robinson AP  et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57:239-47 (1986). Read more (PubMed: 3512425) »

See all 2 Publications for this product

レビューと Q&A

We currently carry three different rat-reactive Abs against CD11b that are raised in rabbit. Please see the link below for a list of these products:

...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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