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Rat peritoneal macrophages
ab33827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.
Our Abpromise guarantee covers the use of ab33827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.
Samples need to be PLP (periodate-lysine-paraformaldehyde) fixed.
|ICC/IF||Use at an assay dependent concentration.|
ab33827 has not yet been referenced specifically in any publications.