製品の概要

  • 製品名Anti-CD11b/c antibody [OX42]
    CD11b/c 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OX42] to CD11b/c
  • 特異性Recognises a common epitope shared by CD11b/c and precipitates three polypeptides of Mw160kD, 103kD and 95kD; a fainter band may also be seen at 133kD (PMID: 3512425). It reacts with all macrophages in the peritoneal cavity, activated macrophages and Kupffer cells, but with only 35% of alveolar macrophages. It also binds to all granulocytes and to dendritic cells, cells with microglial morphology in brain, sparse thymic cortical cells and epidermal Langerhans cells. It inhibits complement mediated rosettes.
  • アプリケーション適用あり: IHC-FoFr, ELISA, Flow Cyt, IHC-Fr, IHC-FrFl, ICC/IF, IPmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Rat
    非交差種: Mouse
  • 免疫原

    Rat macrophages

  • ポジティブ・コントロール
    • IF/ICC: NR8383 cells. IHC-Fr on Rat Spleen.
  • 特記事項

    PMID: 7868861. The antigen is temperature sensitive; we may recommend following the conditions as recommended in this publication. As per publication low temperature paraffin, using 0.5 to 2% paraformaldehyde fixation instead of 4% or using periodate-lysine-paraformaldehyde (PLP) + 0.25% PFA and 0.1% glutaraldehyde might be helpful in detection. In case of any question please do not hesitate to contact technical team at technical@abcam.com.

    The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b/c equivalent antigen.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b/c equivalent antigen.
  • ポリ/モノモノクローナル
  • クローン名OX42
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.

The use of ABC will enable further dilution of the antibody, see Abreview.

ELISA 1/100 - 1/3200.
Flow Cyt 1/25 - 1/200.

(10 µl per million cells).
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/500.
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.
IP Use at an assay dependent concentration. PubMed: 3512425
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
    • 組織特異性Predominantly expressed in monocytes and granulocytes.
    • 関連疾患Systemic lupus erythematosus 6
    • 配列類似性Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Antigen CD11b (p170) antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen like family member C antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11C antibody
      • Cell surface glycoprotein MAC 1 subunit alpha antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3A antibody
      • Integrin alpha M antibody
      • Integrin alpha X antibody
      • Integrin alpha-M antibody
      • Integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • Integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
      • Integrin, alpha X (complement component 3 receptor 4 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • ITGAX antibody
      • Leu M5, alpha subunit antibody
      • Leukocyte adhesion glycoprotein p150,95 alpha chain antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Leukocyte adhesion receptor p150,95 antibody
      • Leukocyte surface antigen p150,95, alpha subunit antibody
      • MAC 1 antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • MO1A antibody
      • Myeloid membrane antigen, alpha subunit antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • OTTHUMP00000163299 antibody
      • p150 95 integrin alpha chain antibody
      • SLEB6 antibody
      see all

    Anti-CD11b/c antibody [OX42] 画像

    • ab1211 staining CD11b/c in rat spleen tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed with formaldehyde and blocked with BSA for 10 minutes at 21°C. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 16 hours. A Biotin-conjugated Goat anti mouse polyclonal (1/300) was used as the secondary antibody.

    • ab1211 stained NR8383 cells. The cells were 100% methanol fixed for 10 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1211 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • ab1211 staining CD11b/c equivalent in rat spleen tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in formaldehyde, blocked with 10% serum for 30 minutes at 24°C, then incubated with ab1211 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/1000 dilution.

      See Abreview

    • ab1211 staining CD11 in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with NGS in 0.3% PBS-TritonX-100 for 60 minutes at 24°C. The sample was incubated with primary antibody (1/300 in NGS in 0.3% PBS-TritonX-100) at 4°C for 42 hours. Ab150111 (1/500) was used as the secondary antibody.

      See Abreview

    Anti-CD11b/c antibody [OX42] (ab1211) 使用論文

    This product has been referenced in:
    • Sun Y  et al. Therapeutic effect of apocynin through antioxidant activity and suppression of apoptosis and inflammation after spinal cord injury. Exp Ther Med 13:952-960 (2017). IHC ; Rat . Read more (PubMed: 28450925) »
    • Kemp KC  et al. Cytokine therapy-mediated neuroprotection in a Friedreich's ataxia mouse model. Ann Neurol N/A:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 28009062) »

    See all 32 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Spleen)
    Permeabilization No
    Specification Spleen
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Dec 21 2016

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Antigen retrieval step None
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Brandon Jun

    Verified customer

    投稿 Dec 17 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Lung)
    Permeabilization No
    Specification Lung
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 May 31 2013

    According to current literature, both CD11b and CD11c are suitable markers for microglia (neuronal macrophages).

    Please see below for a reference:
    http://www.jneuroinflammation.com/content/4/1/26

    However, please note that...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Spleen)
    Specification Spleen
    Fixative Formaldehyde
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 19 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry free floating
    Sample Rat Tissue sections (liver)
    Specification liver
    Username

    Abcam user community

    Verified customer

    投稿 Dec 23 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Tissue sections (Microglia - cell culture)
    Permeabilization No
    Specification Microglia - cell culture
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Aug 25 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (adult liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Specification adult liver
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Oct 15 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Rat Cell (Mixed primary glial cell culture)
    Specification Mixed primary glial cell culture
    Permeabilization No
    Username

    Ms. Nancy Nutile-McMenemy

    Verified customer

    投稿 Mar 16 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Rat Cell (Primary Mixed Cortical Cultures)
    Specification Primary Mixed Cortical Cultures
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Ms. Nancy Nutile-McMenemy

    Verified customer

    投稿 Nov 09 2005

    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"