Anti-CD11b 抗体 [M1/70] (PE/Cy5®) (ab25533)

製品の概要

  • 製品名Anti-CD11b antibody [M1/70] (PE/Cy5®)
    CD11b 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M1/70] to CD11b (PE/Cy5®)
  • 標識PE/Cy5®. Ex: 496nm, Em: 670nm
  • 特異性ab25533 recognises aM subunit of the CD11b/CD18 heterodimer
  • アプリケーション適用あり: Flow Cyt, Blocking, IHC-Fr, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Dog, Human, Pig, Monkey
  • 免疫原

    Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.

  • 特記事項This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.3µg for 106 cells.
Blocking Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 組織特異性Predominantly expressed in monocytes and granulocytes.
  • 関連疾患Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [M1/70] (PE/Cy5®) 画像

  • ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.

    See Abreview

  • Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.
  • ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live Macrophages.

    See Abreview

  • Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD11b with ab25533 at 0.1 μg/106 cells dilution. A Rat Anti-Mouse Ly-6G/Ly-6C-PE was used as the secondary antibody.

Anti-CD11b antibody [M1/70] (PE/Cy5®) (ab25533) 使用論文

This product has been referenced in:
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Read more (PubMed: 25936350) »
  • Vrecenak JD  et al. Stable long-term mixed chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation. Blood 124:1987-95 (2014). Read more (PubMed: 24869940) »

See all 3 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Fixation None
Permeabilization No
Sample Sheep Cell (Peripheral blood)
Specification Peripheral blood
Gating Strategy Monocytes
Preparation Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation None
Permeabilization No
Sample Human Cell (Peripheral blood)
Specification Peripheral blood
Gating Strategy Granulocytes
Preparation Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

投稿 Sep 11 2013

Application Flow Cytometry
Fixation None
Permeabilization No
Sample Cow Cell (Peripheral blood)
Specification Peripheral blood
Gating Strategy Monocytes
Preparation Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

投稿 Aug 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
Sample buffer: PBS
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

投稿 Feb 19 2013

Protein G is used for purification of this antibody.

Ab25533 Anti-CD11b antibody [M1/70] (PE/Cy5®) is purified, conjugated, and then further purified to remove free antibody and conjugates.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

投稿 Dec 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rabbit Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

投稿 Dec 20 2012

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen from immune mouse)
Specification Spleen from immune mouse
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 25 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"