製品の概要

  • 製品名Anti-CD11b antibody [M1/70] (FITC)
    CD11b 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M1/70] to CD11b (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, Blocking, IPmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rabbit, Human
  • 免疫原

    Tissue/ cell preparation (Mouse): C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes.

  • ポジティブ・コントロール
    • BLAB/c spleen cells.

アプリケーション

Our Abpromise guarantee covers the use of ab24874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 組織特異性Predominantly expressed in monocytes and granulocytes.
  • 関連疾患Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [M1/70] (FITC) 画像

  • ab24874 at 1 µg/106 cells staining BALB/c splenocytes. Cells were then double stained with Rat monoclonal [RB6-8C5] to Ly6g (Phycoerythrin) (ab24884).  Lymphocytes were gated and analyzed by flow cytometry.

  • Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD11b with ab24874 at 1 μg/106 cells dilution. A Rat Anti-Mouse Ly-6G/Ly-6C-PE was used as the secondary antibody.

Anti-CD11b antibody [M1/70] (FITC) (ab24874) 使用論文

This product has been referenced in:
  • Liu T  et al. Gr-1+CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation. Sci Rep 4:4833 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24776637) »
  • Gil M  et al. Surfactant Protein A Modulates Cell Surface Expression of CR3 on Alveolar Macrophages and Enhances CR3-mediated Phagocytosis. J Biol Chem 284:7495-504 (2009). Flow Cyt . Read more (PubMed: 19155216) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (liver)
Specification liver
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative OCT
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投稿 Dec 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (splenocytes)
Specification splenocytes
Preparation Cell harvesting/tissue preparation method: cell suspension
Sample buffer: PBS
Permeabilization No
Gating Strategy Live cells
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投稿 May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"