製品の概要

  • 製品名Anti-CD11b antibody [M1/70]
    CD11b 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M1/70] to CD11b
  • アプリケーション適用あり: ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rabbit, Human
  • 免疫原

    The hybridoma was formed by the fusion of mouse myeloma NS1 cellswith spleen cells from rats immunized with B10 mouse spleen cells enrichedfor T lymphocytes.

  • ポジティブ・コントロール
    • Flow cytometry: Live macrophages and Raw246.7cells. IF/ICC: Raw246.7 cells.
  • 特記事項

    CD11b is also known as MAC-1alpha, CR3 and integrin alpha M.  We do not batch test ab8878 in IHC, however, some customers have successfully used this antibody in this application. We cannot recommend this antibody for IHC.

    Alternative versions available:
    Anti-CD11b antibody (Alexa Fluor® 405) [M1/70] (ab197703)
    Anti-CD11b antibody(Alexa Fluor® 488) [M1/70] (ab197701)

    Anti-CD11b antibody (Alexa Fluor® 647) [M1/70] (ab197702)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration.

Each labeling step was carried out for 30 min at 0-4°C in mediumcontaining 0.01 M NaN3. Cells (5 x 107/ml) were incubated with 50 µl M1 monoclonal antibody or irrelevant monoclonal antibody, R4/18.2, as control in the first step, washed, suspended in 50 µl of FITC-F (ab')2 anti-rat IgGin the second step, and washed through a layer of fetal calf serum. Can also be used in cytotoxicity and binding assays.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 組織特異性Predominantly expressed in monocytes and granulocytes.
  • 関連疾患Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [M1/70] 画像

  • ICC/IF image of ab8878 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8878 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing Human macrophages stained with ab8878 (blue line). Cells were incubated with human immunoglobulin for 30 min at 4°C to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8878, 20 µg/mL) for 30 min at 4ºC. The secondary antibody used was a mouse anti-rat FITC (IgG2b gamma chain) (ab99671) at 1 µg/mL for 30 min at 4ºC. Isotype control antibody (black line) was Rat IgG2b [RTK4530] (ab18541) used under the same conditions.

  • Overlay histogram showing Human macrophages stained with ab8878 (red line). Cells were pre-incubated in Human AB serum (10%) for 20 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CD11b antibody [M1/70] (ab8878) 使用論文

This product has been referenced in:
  • Arroba AI  et al. Autophagy resolves early retinal inflammation in Igf1-deficient mice. Dis Model Mech 9:965-74 (2016). WB ; Mouse . Read more (PubMed: 27483352) »
  • Li C  et al. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix. Nat Commun 7:11455 (2016). IHC (PFA fixed) . Read more (PubMed: 27126736) »

See all 23 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse primary microglia cells)
Permeabilization Yes - 0.5% TX100
Specification mouse primary microglia cells
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Oct 10 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 17 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Tissue sections (whole mouse embryo)
Specification whole mouse embryo
Permeabilization Yes - 0.1% triton x100 in TBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 31 2013

Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.
...

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Application Immunocytochemistry
Sample Mouse Cell (RAW264.7 cell line)
Specification RAW264.7 cell line
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2012

Thank you for your reply.

The secondary antibody I recommended, ab7093, will react with primary antibodies raised in rat. It has also been prepared so that it will not react with antibodies or samples from rabbit or mouse. This is desirable f...

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Thank you for contacting us.

You can find our secondary antibody search tool here:

http://www.abcam.com/index.html?pageconfig=productmap&cl=918

For ab8878, I would recommend using the secondary ab7093. This antibody is...

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Thank you very much for your call today.

Sinceab4055 has not been tested with mouse samples, I can offer a discount off a future purchase if you buy ab4055 now, test it with mouse samples and submit feedback to us in the form of an Abreview. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW cell line)
Specification RAW cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Aug 01 2012

Thank you for contacting us and your interest in our products.

To produce the anti-CD11b http://www.abcam.com/ab8878, spleen cells from a B10 mouse were injected into a rat.The mouse spleen cells were enriched for T-lymphocytes prior to inject...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"