Anti-CD11b 抗体 [DCIS1/18] (PerCP) (ab91145)

製品の概要

  • 製品名Anti-CD11b antibody [DCIS1/18] (PerCP)
    CD11b 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DCIS1/18] to CD11b (PerCP)
  • 標識PerCP. Ex: 482nm, Em: 675nm
  • 特異性Integrin heterodimer of MW 165,95kD which comprises the receptor (CR3 am chain, CD11b/ CD18) for the complement component C3i. The CR3 receptor is expressed as a surface molecule by circulating monocytes and granulocytes and also by dendritic reticulum cells in sections of lympoid tissue. Expression is increased on activated granulocytes.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human CD11b. Immunogen: Dendritic cells derived from human monocytes.

アプリケーション

Our Abpromise guarantee covers the use of ab91145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 20µl for 106 cells. ab110422-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 組織特異性Predominantly expressed in monocytes and granulocytes.
  • 関連疾患Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [DCIS1/18] (PerCP) 画像

  • ab91145 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.

    See Abreview

Anti-CD11b antibody [DCIS1/18] (PerCP) (ab91145) 使用論文

This product has been referenced in:
  • Hams E  et al. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis. Proc Natl Acad Sci U S A 111:367-72 (2014). Read more (PubMed: 24344271) »
  • Xue L  et al. Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells. J Allergy Clin Immunol 133:1184-94 (2014). Flow Cyt ; Human . Read more (PubMed: 24388011) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rabbit Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

投稿 Dec 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

投稿 Dec 21 2012

Thank you for contacting us. To our knowledge, the epitope for ab91145 has not been mapped, but the immunogen region shows ˜70% homology between mouse and human. Our customers have previously reported poor results using this antibodyin mouse, rat, monk...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive macrophages
Username

Abcam user community

Verified customer

投稿 Apr 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy neutrophils
Username

Abcam user community

Verified customer

投稿 Aug 25 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Blood cells)
Specification Blood cells
Preparation Cell harvesting/tissue preparation method: Lysis of red blood cells
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy Neutrophils
Username

Abcam user community

Verified customer

投稿 Dec 21 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Monkey Cell (Blood cells)
Specification Blood cells
Preparation Cell harvesting/tissue preparation method: Lysis of red blood cells
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy Monocytes
Username

Abcam user community

Verified customer

投稿 Dec 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (leukocytes)
Specification leukocytes
Preparation Cell harvesting/tissue preparation method: staining after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy neutrophils
Username

Abcam user community

Verified customer

投稿 Aug 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"