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Our Abpromise guarantee covers the use of ab8879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
NR8383, rat macrophage cells, were used for a flow cytometry analysis with ab8879 (CD11b) antibody that binds the rat Mac-1 alpha subunit (CD11b). Cells were treated with ab8879 at a 1:50 dilution and subsequently with anti-murine IgG FITC. A 94% shift is seen as compared to the non-treated control.
IHC - Wholemount of rat retina tissue labelling CD11b (white) with ab8879. Sample was incubated with primary antibody (1/100 in PBS) for 24 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody. Blue - DAPI.
ab8879 staining CD11b in mixed rat spinal cord cultures on astrocytes by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were live stained. Samples were incubated with primary antibody (1/200 in DMEM) for 20 minutes. An Alexa Fluor®488-conjugated Rat anti-rabbit IgG1 monoclonal (1/600) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"