製品の概要

  • 製品名Anti-CD11b antibody
    CD11b 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CD11b
  • アプリケーション適用あり: IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-RNDGEDSYRTQ

    , corresponding to internal sequence amino acids 807-817 of Human CD11b

  • ポジティブ・コントロール
    • Human Brain (Cerebellum) lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 19071955
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

ターゲット情報

  • 機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 組織特異性Predominantly expressed in monocytes and granulocytes.
  • 関連疾患Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody 画像

  • Anti-CD11b antibody (ab62817) at 0.3 µg/ml + Human Brain (Cerebellum) lysate (in RIPA buffer) at 35 µg/ml

    Predicted band size : 127 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
  • ab62817 (2µg/ml) staining of paraffin embedded Human Brain shows staining of microglia following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining.

  • ab62817 staining CD11b in mouse retina tissue sections by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 3% BSA at room temperature for 40 minutes. Samples were incubated with primary antibody (1/200 in 1% BSA) for 24 hours at 4°C. An AlexaFluor® 488-conjugated donkey anti-goat polyclonal (1/150) was used as the secondary antibody.

    See Abreview

Anti-CD11b antibody (ab62817) 使用論文

This product has been referenced in:
  • Zhang R  et al. Helium preconditioning protects mouse liver against ischemia and reperfusion injury through the PI3K/Akt pathway. J Hepatol 61:1048-55 (2014). Read more (PubMed: 24972044) »
  • Lees CW  et al. Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways. PLoS Med 5:e239 (2008). IHC-Fr ; Human . Read more (PubMed: 19071955) »

See all 2 Publications for this product

Product Wall

We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

ab...

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Merci de votre intérêt pour les anti-CD11b d'Abcam.

Anotre connaissance :
1) l'anticorps ab62817n’a pas encore été testéchez la souris (90% d'homologie entre l'immunogène et le CD11b de la s...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen)
Permeabilization Yes - 0.1% TritonX-Tween
Specification Spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 May 24 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Aug 13 2012

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse retina)
Specification mouse retina
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: RT°C
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投稿 Jun 27 2012

Thank you for your email.

CD271: We have ab8877 which is fully tested with Sheep samples and is guaranteed to work.

CD34: ab963, ab8536, ab30375, ab30377 are the sheep specific antibodies.

CD11b: It seems ab75476, ab62817...

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Thank you for submitting your Abreview and for your honest feedback.
As you had rated your experience in the review as 'Below average', I just wanted to follow up with you to get some more details as to what you were unhappy about regarding use of...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 37°C
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投稿 Apr 03 2012

Code de remise : ********
Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participerà notre offre et tester ab62817 chez la souris. Le code de réduction correspondant est *******. Ce code de ré...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (substantia nigra tissue lysate)
Loading amount 60 µg
Specification substantia nigra tissue lysate
Treatment Rat substantia nigra injected with LPS or saline
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Dec 28 2011

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"