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Tissue/ cell preparation (Human). Peripheral blood lymphocytes.
Our Abpromise guarantee covers the use of ab3981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration. PubMed: 23039186|
|Flow Cyt||Use a concentration of 1 µg/ml.|
|Functional Studies||Use at an assay dependent concentration. The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.|
|IP||Use at an assay dependent concentration.|