製品の概要

  • 製品名Anti-CD11a antibody
    CD11a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD11a
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CD11a.

    (Peptide available as ab90248.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human thymus; Human spleen.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab89725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 129 kDa).

ターゲット情報

  • 機能Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • 組織特異性Leukocytes.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Lymphocyte function associated antigen, type 1, alpha subunit antibody
    • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
    • Antigen CD11A antibody
    • CD 11a antibody
    • CD11 antigen-like family member A antibody
    • CD11a antibody
    • CD11a antigen antibody
    • Integrin Alpha L antibody
    • Integrin alpha-L antibody
    • Integrin gene promoter antibody
    • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
    • ITAL_HUMAN antibody
    • Itgal antibody
    • ITGAL protein antibody
    • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
    • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
    • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
    • Leukocyte function associated molecule 1 alpha chain antibody
    • Leukocyte function-associated molecule 1 alpha chain antibody
    • LFA 1 alpha (LFA1A) antibody
    • LFA 1 alpha antibody
    • LFA 1 antibody
    • LFA 1A antibody
    • LFA-1A antibody
    • LFA1A antibody
    • Ly15 antibody
    • Ly21 antibody
    • Lymphocyte function associated antigen 1 antibody
    • Lymphocyte Function Associated Antigen Type 1 alpha antibody
    • lymphocyte function-associated antigen 1, alpha polypeptide antibody
    • p180 antibody
    see all

Anti-CD11a antibody 画像

  • All lanes : Anti-CD11a antibody (ab89725) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 129 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • IHC image of CD11a staining in FFPE human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89725, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CD11a antibody (ab89725) 使用論文

ab89725 has not yet been referenced specifically in any publications.

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