製品の概要

  • 製品名Anti-CD105 antibody
    CD105 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD105
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human CD105 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P17813

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human Heart and Kidney tissue lysates as well as HUVEC whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).

ターゲット情報

  • 機能Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 組織特異性Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 関連疾患Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody 画像

  • All lanes : Anti-CD105 antibody (ab124610) at 1 µg/ml

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 2 : HUVEC (Human Umbilical vein endothelial cell line) Whole Cell Lysate
    Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    CD105 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    The predicted molecular weight of CD105 is 70 kDa (SwissProt), however we expect to observe a banding pattern at 90 kDa.

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124610 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-CD105 antibody (ab124610) 使用論文

ab124610 has not yet been referenced specifically in any publications.

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