製品の概要

  • 製品名Anti-CD105 antibody
    CD105 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD105
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide from C-terminal residues of Human CD105.

  • ポジティブ・コントロール
    • Human Fetal Lung and MCF7 cell lysates, Human Fetal Small Intestine tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 組織特異性Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 関連疾患Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody 画像

  • ab107595 staining CD105 in Rat heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Membrane staining of formalin-fixed paraffin-embedded Human Fetal Small Intestine with ab107595 at a dilution of 1/100.
  • All lanes : Anti-CD105 antibody (ab107595) at 1/500 dilution

    Lane 1 : Human Fetal Lung lysate
    Lane 2 : MCF7 cell lysate


    Predicted band size : 71 kDa

Anti-CD105 antibody (ab107595) 使用論文

This product has been referenced in:
  • Kawasaki T  et al. Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation. Dev Growth Differ N/A:N/A (2016). IF . Read more (PubMed: 27241908) »
  • Chadchan SB  et al. Endoglin (CD105) coordinates the process of endometrial receptivity for embryo implantation. Mol Cell Endocrinol 425:69-83 (2016). Read more (PubMed: 26802878) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step None
Sample Human Tissue sections (synovium)
Specification synovium
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Aug 05 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step None
Sample Mouse Tissue sections (synovium)
Specification synovium
Permeabilization No
Fixative Zinc buffered formalin
Username

Mrs. Merissa Olmer

Verified customer

投稿 Aug 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Heart Cells)
Specification Heart Cells
Fixative Formaldehyde
Permeabilization Yes - Triton X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 10 2013

Thank you for getting back to me with your most recent results.

I am sorry to hear that the protocol suggestions have not improved the results. I can understand your frustration and your reluctance to optimise any further. I believe with the ...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided by you will enable us to investigate this case and will provide us wi...

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D'accord, pas de problème. Merci pour cette information.

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Merci de votre intérêt pour ab107595.

Comme expliqué par téléphone, cet anticorps n’a pas encore été testé en cytométrie en flux et nous n’offrons pas d’éc...

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Thank you for submitting an Abreview of ab107595. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lymph node)
Specification lymph node
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

投稿 May 25 2012

DISCOUNT CODE:42XXXXX Expiration date: 20/04/2012 I am very pleased to hear you would like to accept our offer and test ab107595 in Rat in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"