Anti-CD10 抗体 [EPR2997] (ab79423)


  • 製品名Anti-CD10 antibody [EPR2997]
    CD10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2997] to CD10
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N terminal of Human CD10.

  • ポジティブ・コントロール
    • Jurkat and LnCap lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab79423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
Flow Cyt 1/100 - 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
    • 配列類似性Belongs to the peptidase M13 family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Atriopeptidase antibody
      • CALLA antibody
      • CD10 antibody
      • CD10 antigen antibody
      • Common acute lymphocytic leukemia antigen antibody
      • DKFZp686O16152 antibody
      • EC antibody
      • Enkephalinase antibody
      • EPN antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
      • Membrane metallo endopeptidase antibody
      • Membrane metallo endopeptidase variant 1 antibody
      • Membrane metallo endopeptidase variant 2 antibody
      • Membrane metalloendopeptidase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
      • Membrane metalloendopeptidase variant 1 antibody
      • Membrane metalloendopeptidase variant 2 antibody
      • MGC126681 antibody
      • MGC126707 antibody
      • MME antibody
      • NEP antibody
      • NEP_HUMAN antibody
      • Neprilysin antibody
      • neprilysin-390 antibody
      • neprilysin-411 antibody
      • Neutral endopeptidase 24.11 antibody
      • Neutral endopeptidase antibody
      • Neutral endopeptidase, membrane-associated antibody
      • SFE antibody
      • Skin fibroblast elastase antibody
      see all

    Anti-CD10 antibody [EPR2997] 画像

    • All lanes : Anti-CD10 antibody [EPR2997] (ab79423) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : LnCap cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 86 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing Jurkat cells stained with ab79423 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79423, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    Anti-CD10 antibody [EPR2997] (ab79423) 使用論文

    ab79423 has not yet been referenced specifically in any publications.

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    Thank you or your inquiry.
    I can confirm that ab79423 is a rabbit monoclonal antibody of the IgG isotype.
    Unlike mice, rabbits do not have sub-isotypes like IgG2a for example.
    I recommend to choose a isotype control that also is monoclon...

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