Anti-CD10 抗体 [EPR2997] (ab79423)
製品の概要
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製品名Anti-CD10 antibody [EPR2997]
CD10 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2997] to CD10
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由来種Rabbit
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アプリケーション適用あり: WB, Flow Cytmore details
適用なし: IP -
種交差性交差種: Mouse, Rat, Human
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免疫原
A synthetic peptide corresponding to residues on the N terminal of Human CD10.
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ポジティブ･コントロール
- Jurkat and LnCap lysates.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
精製度Protein A purified
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ポリ/モノモノクローナル
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クローン名EPR2997
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab79423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa). | |
| Flow Cyt | 1/100 - 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
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配列類似性Belongs to the peptidase M13 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Entrez Gene: 24590 Rat
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- SwissProt: P07861 Rat
- Unigene: 307734 Human
see all -
別名
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
画像
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![Western blot - Anti-CD10 antibody [EPR2997] (ab79423)](http://a.abcam.com/ps/products/79/ab79423/Images/CD10-Primary-antibodies-ab79423-1.jpg)
Western blot - Anti-CD10 antibody [EPR2997] (ab79423)All lanes : Anti-CD10 antibody [EPR2997] (ab79423) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : LnCap cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?) -
![Flow Cytometry - Anti-CD10 antibody [EPR2997] (ab79423)](http://a.abcam.com/ps/products/79/ab79423/Images/CD10-Primary-antibodies-ab79423-2.jpg)
Flow Cytometry - Anti-CD10 antibody [EPR2997] (ab79423)Overlay histogram showing Jurkat cells stained with ab79423 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79423, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
参考文献
This product has been referenced in:
- Kukreja L et al. Increased mtDNA mutations with aging promotes amyloid accumulation and brain atrophy in the APP/Ld transgenic mouse model of Alzheimer's disease. Mol Neurodegener 9:16 (2014). WB . Read more (PubMed: 24885175) »
- Sadleir KR et al. Elevated Aß42 in aged, non-demented individuals with cerebral atherosclerosis. Curr Alzheimer Res 10:785-9 (2013). Read more (PubMed: 23919773) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"