製品の概要

  • 製品名Anti-CCT6A antibody
    CCT6A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CCT6A
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of Human CCT6A.

  • ポジティブ・コントロール
    • Human breast carcinoma tissue; HepG2 cell extracts This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab110905 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性CCT6A is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of CCT6A, encoding different isoforms, have been characterized.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • Acute morphine dependence related protein 2 antibody
    • Amino acid transport defect complementing antibody
    • CCT zeta 1 antibody
    • CCT zeta antibody
    • CCT-zeta antibody
    • CCT-zeta-1 antibody
    • CCT6 antibody
    • CCTZ antibody
    • Chaperonin containing T complex subunit 6 antibody
    • Chaperonin containing TCP1 subunit 6A antibody
    • Chaperonin containing TCP1 subunit 6A (zeta 1) antibody
    • Histidine transport regulator 3 antibody
    • HTR3 antibody
    • MGC126214 antibody
    • MGC126215 antibody
    • MoDP 2 antibody
    • MoDP-2 antibody
    • MoDP2 antibody
    • T complex protein 1 subunit zeta antibody
    • T complex protein 1 zeta subunit antibody
    • TCP 1 zeta antibody
    • TCP-1-zeta antibody
    • TCP1 zeta antibody
    • TCP20 antibody
    • TCPZ antibody
    • TTCP20 antibody
    see all

Anti-CCT6A antibody 画像

  • ICC/IF image of ab110905 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110905 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab110905, at a 1/50 dilution, staining CCT6A in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is from tissue treated with the synthesized peptide.
  • All lanes : Anti-CCT6A antibody (ab110905) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HepG2 cell extracts with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa

Anti-CCT6A antibody (ab110905) 使用論文

ab110905 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"