製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CCRL2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (LSAQLVPSLC SAVFVIGVLD NLLVVLILVK YKGLKRVENI YLLNLAVSNL) of Human CCRL2 (NP_003956).

  • ポジティブ・コントロール
    • 721_B Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Receptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses.
  • 組織特異性
    Expressed abundantly in immunal tissues such as spleen, fetal liver, lymph node and bone marrow. Strong expression also in lung and heart. Expressed in almost all hematopoietic cells including monocytes, macrophages, PMNs, T-cells (both CD4+ and CD8+), monocyte-derived iDCs, NK cells, and CD34+ progenitor cells. B cells expressed isoform 1 but not isoform 2. Up-regulated on synovial neutrophils of rheumatoid arthritis patients.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • ドメイン
    Lacks the conserved DRYLAIV motif in the second intracellular loop that is required for signaling of functional chemokine receptors.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-C chemokine receptor-like 2 antibody
    • CCRL2 antibody
    • CCRL2_HUMAN antibody
    • Chemokine (C-C motif) receptor like 2 antibody
    • Chemokine receptor CCR11 antibody
    • Chemokine receptor X antibody
    • CKRX antibody
    • CRAM A antibody
    • CRAM B antibody
    • CRAMA antibody
    • CRAMB antibody
    • FLJ55815 antibody
    • HCR antibody
    • MGC116710 antibody
    • MGC34104 antibody
    • Putative MCP 1 chemokine receptor antibody
    • Putative MCP-1 chemokine receptor antibody
    see all

画像

  • Anti-CCRL2 antibody (ab85224) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : ~39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~70 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab85224 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (endometrium, myometrium, pituitary, brain)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
30 µg
Specification
endometrium, myometrium, pituitary, brain
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Marta Kiezun

Verified customer

投稿 Nov 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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