Anti-CCR9 抗体 [CW-1.2] (Phycoerythrin) (ab95671)

製品の概要

  • 製品名
    Anti-CCR9 antibody [CW-1.2] (Phycoerythrin)
    CCR9 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CW-1.2] to CCR9 (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    WT Mouse thymocytes (Eur J Immunol. 2006 Jan;36(1):73-81)

  • ポジティブ・コントロール
    • Mouse C57BL/6 thymocytes

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% Gelatin, PBS(containing horse serum) pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    CW-1.2
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.

ターゲット情報

  • 関連性
    The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
  • 細胞内局在
    Cell membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • C C chemokine receptor D6 antibody
    • C C chemokine receptor type 9 antibody
    • C C CKR 9 antibody
    • CC Chemokine receptor CCR10 antibody
    • CC CKR 9 antibody
    • CCBP2 antibody
    • CCCKR9 antibody
    • CCR 9 antibody
    • CCR9 antibody
    • CDw199 antibody
    • CDw199 antigen antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
    • Chemokine (C-C motif) receptor 9 isoform A antibody
    • Chemokine (C-C motif) receptor 9 isoform B antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 10 antibody
    • Chemokine CC Motif Receptor 9 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine-binding protein D6 antibody
    • Cmkbr10 antibody
    • CMKBR9 antibody
    • G protein coupled receptor 28 antibody
    • GPR 28 antibody
    • GPR 9 6 antibody
    • GPR28 antibody
    • GPR96 antibody
    • OTTHUMP00000164653 antibody
    • OTTHUMP00000164654 antibody
    see all

画像

  • Flow cytometry staining of C57BL/6 thymocytes with Anti-Mouse CD8a APC and 0.125 µg of Mouse IgG2a? Isotype Control PE (left) or 0.25 µg ab95671 (right). Total viable cells were used for analysis.

プロトコール

参考文献

This product has been referenced in:
  • Wurbel MA  et al. Complex regulation of CCR9 at multiple discrete stages of T cell development. Eur J Immunol 36:73-81 (2006). Flow Cyt . Read more (PubMed: 16342233) »

See 1 Publication for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

投稿 Nov 06 2012

Thank you for submitting an Abreview of ab95671. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (human), we would like to follow up on this to s...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: from buffy coat after ficoll preparation
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 24 2012

Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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