製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to CCR9
  • 特異性
    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
  • アプリケーション
    適用あり: IHC-FoFr, Flow Cyt, ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide:

    IPGMFDDFSYDSTASTDDYMNLNFSSFF

    , corresponding to amino acids 10-37 of Mouse CCR9.

  • ポジティブ・コントロール
    • Mouse thymus or lymphnodes.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    PBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/500 - 1/1000. After perfusion place in 30% sucrose overnight.
Flow Cyt 1/200.
ELISA 1/100000.
IHC-P 1/250.
WB 1/1000.

ターゲット情報

  • 関連性
    The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
  • 細胞内局在
    Cell membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • C C chemokine receptor D6 antibody
    • C C chemokine receptor type 9 antibody
    • C C CKR 9 antibody
    • CC Chemokine receptor CCR10 antibody
    • CC CKR 9 antibody
    • CCBP2 antibody
    • CCCKR9 antibody
    • CCR 9 antibody
    • CCR9 antibody
    • CDw199 antibody
    • CDw199 antigen antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
    • Chemokine (C-C motif) receptor 9 isoform A antibody
    • Chemokine (C-C motif) receptor 9 isoform B antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 10 antibody
    • Chemokine CC Motif Receptor 9 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine-binding protein D6 antibody
    • Cmkbr10 antibody
    • CMKBR9 antibody
    • G protein coupled receptor 28 antibody
    • GPR 28 antibody
    • GPR 9 6 antibody
    • GPR28 antibody
    • GPR96 antibody
    • OTTHUMP00000164653 antibody
    • OTTHUMP00000164654 antibody
    see all

画像

  • Immonuhistochemistry on mouse spleen, at 1/250 dilution.

参考文献

This product has been referenced in:
  • Li J  et al. Anti-CCL25 antibody prolongs skin allograft survival by blocking CCR9 expression and impairing splenic T-cell function. Arch Immunol Ther Exp (Warsz) 61:237-44 (2013). Read more (PubMed: 23456208) »
  • Deutsch AJ  et al. Chemokine receptors in gastric MALT lymphoma: loss of CXCR4 and upregulation of CXCR7 is associated with progression to diffuse large B-cell lymphoma. Mod Pathol : (2012). IHC ; Human . Read more (PubMed: 22936065) »

See all 4 Publications for this product

レビューと Q&A

Thank you for your patience. The originator of ab12953 and ab13396 has gotten back to me with the standard IHC protocol that they follow which I have included below. If you have any additional questions, please let me know. Tissue Preparation: ...

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Thank you for your phone call. For ab13268, ab1662, and ab1669, the originator has informed me that the antigen retrieval method for IHC-formalin fixed, paraffin embedded sections involved treating the sections with antigen retrieval solution (Dako # S...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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