製品の概要

  • 製品名Anti-CCR7 antibody [Y59]
    CCR7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y59] to CCR7
  • 特異性The antibody does not cross-react with other G-protein coupled receptor 1 family members.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CCR7 (N terminal).
    Database link: P32248

  • ポジティブ・コントロール
    • WB: K562 and Daudi cell lysates. IHC-P: human spleen tissue. ICC/IF: HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:
    Anti-CCR7 antibody (Alexa Fluor® 488) [Y59] (ab196459)
    Anti-CCR7 antibody (Alexa Fluor® 647) [Y59] (ab196640)
    Anti-CCR7 antibody (HRP) [Y59] (ab196475)
    Anti-CCR7 antibody (Alexa Fluor® 594) [Y59] (ab207016)
    Anti-CCR7 antibody (Alexa Fluor® 405) [Y59] (ab207017)
    Anti-CCR7 antibody (Alexa Fluor® 555) [Y59] (ab207018)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with CCR7 peptide (ab209386).

For unpurified use at 1/5000.

IHC-P 1/1000.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100.

For unpurified use at 1/250.

IP 1/20.

For unpurified use at 1/10.

ターゲット情報

  • 機能Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • 組織特異性Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BLR 2 antibody
    • BLR2 antibody
    • C C chemokine receptor type 7 antibody
    • C C CKR 7 antibody
    • C-C chemokine receptor type 7 antibody
    • C-C CKR-7 antibody
    • CC chemokine receptor 7 antibody
    • CC chemokine receptor type 7 antibody
    • CC CKR 7 antibody
    • CC-CKR-7 antibody
    • CCCKR7 antibody
    • CCR 7 antibody
    • CCR-7 antibody
    • Ccr7 antibody
    • CCR7_HUMAN antibody
    • CD 197 antibody
    • CD197 antibody
    • CD197 antigen antibody
    • CDw197 antibody
    • Chemokine (C C motif) receptor 7 antibody
    • Chemokine (C C) receptor 7 antibody
    • Chemokine C C motif receptor 7 antibody
    • Chemokine C C receptor 7 antibody
    • Chemokine receptor 7 antibody
    • Chemokine receptor 7-like protein antibody
    • CMKBR7 antibody
    • EBI 1 antibody
    • EBI1 antibody
    • Ebi1h antibody
    • EBV Induced G Protein Coupled Receptor 1 antibody
    • EBV-induced G-protein coupled receptor 1 antibody
    • Epstein Barr virus induced G protein coupled receptor antibody
    • Epstein Barr virus induced gene 1 antibody
    • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
    • MGC108519 antibody
    • MIP 3 beta receptor antibody
    • MIP-3 beta receptor antibody
    • MIP3 Beta Receptor antibody
    see all

Anti-CCR7 antibody [Y59] 画像

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : COS-1 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : RAW264.7 cell lysate
    Lane 4 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 43 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • ab32527 staining CCR7 in Mouse CT26-WT tumor tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% goat serum for 60 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/10000 in blocking buffer) for 20 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (10 µg/mL) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 43 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : Daudi cell lysate


    Predicted band size : 43 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF-7 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CCR7 with purified ab32527 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen labelling CCR7 with unpurified ab32527 at 1/250.

    Immunohistochemical analysis of paraffin-embedded human spleen using ab32527 at 1/250 dilution.
  • Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CCR7 with unpurified ab32527 at 1/250.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CCR7 with unpurified ab32527 at 1/1000. Cells were fixed with 4% PFA fixed (10 min) and permeabilized and blocked with 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (shown in green). Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

Anti-CCR7 antibody [Y59] (ab32527) 使用論文

This product has been referenced in:
  • Pang MF  et al. TGF-ß1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Oncogene N/A:N/A (2015). WB, IHC-P, IF ; Mouse, Human . Read more (PubMed: 25961925) »
  • Guo J  et al. Effect of CCR7, CXCR4 and VEGF-C on the lymph node metastasis of human pancreatic ductal adenocarcinoma. Oncol Lett 5:1572-1578 (2013). IHC-P ; Human . Read more (PubMed: 23761820) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (CT26-WT tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization No
Specification CT26-WT tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Miss. Sylvie Colin

Verified customer

投稿 Jun 14 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Sample Rat Tissue sections (nerve)
Specification nerve
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 11 2013

Thank you very much for contacting us.

Rabbit monoclonal antibodies have very high affinity for their antigens, so they are often used more diluted than a comparable mouse monoclonal antibody would be. We don't have any data for htis antibody...

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Thanks for the email.

I'm happy to provide the concentration. It's lot specific so when you have a chance please provide the lot number for that vial of ab32527.

Thanks!

Thanks for your email.

According to information I have this product should arrive as a liquid (100ul). I'm sorry to ask a rather silly question here but did you centrifuge the vial prior to opening, in case all the liquid is in the cap?
...

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Thanks for your email.

I hope you've been able to claim your free antibody. If not, please note I have added your discount code to your review for ab32527 so you should have no problem using that code to get your free primary now.

I...

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Application Flow Cytometry
Sample Rat Cell (primary cortical cultures)
Specification primary cortical cultures
Fixation none
Permeabilization No
Gating Strategy live cells (no PI uptake)
Username

Abcam user community

Verified customer

投稿 Aug 25 2012


I am very pleased to hear you would like to accept our offer and test ab32527 in flow cytometry. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an A...

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Thank you for your enquiry.

I've looked and have not been able to find any information regarding flow cytometry testing and ab32527. It may be that this note on our datasheet is an error.

If you'd like to test this antibody in flow ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 6.0
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 37°C
Username

Mr. Peter Sullivan

Verified customer

投稿 Jun 21 2012

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"