This antibody gave a positive signal in Human Spleen tissue lysate as well as K562 whole cell lysate.
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Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
MIP 3 beta receptor antibody
MIP-3 beta receptor antibody
MIP3 Beta Receptor antibody
Western blot - Anti-CCR7 antibody (ab125205)
All lanes : Anti-CCR7 antibody (ab125205) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699) Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa Observed band size: 42 kDa Additional bands at: 45 kDa, 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125205 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.