Anti-CCR5 抗体 [HEK/1/85a] (FITC) (ab11466)

製品の概要

  • 製品名
    Anti-CCR5 antibody [HEK/1/85a] (FITC)
    CCR5 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [HEK/1/85a] to CCR5 (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    This antibody recognises human CCR5.
  • アプリケーション
    適用あり: IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    CHO cells transfected with human CCR5.

  • 特記事項
    This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
Flow Cyt
  • 追加情報
    Flow Cyt: neat - 1/10.
    Use 10µl of the suggested working dilution to label 100µl fresh whole blood.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
    • 組織特異性
      Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
    • 関連疾患
      Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • 配列類似性
      Belongs to the G-protein coupled receptor 1 family.
    • 翻訳後修飾
      Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
      O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
      Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
      Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AM4 7 antibody
      • C C chemokine receptor type 5 antibody
      • C C CKR 5 antibody
      • C-C chemokine receptor type 5 antibody
      • C-C CKR-5 antibody
      • C-C motif chemokine receptor 5 A159A antibody
      • CC Chemokine Receptor 5 antibody
      • CC Chemokine Receptor Type 5 antibody
      • CC CKR 5 antibody
      • CC-CKR-5 antibody
      • CCCKR 5 antibody
      • CCCKR5 antibody
      • CCR 5 antibody
      • CCR-5 antibody
      • CCR5 antibody
      • CCR5 chemokine (C C motif) receptor 5 antibody
      • CCR5_HUMAN antibody
      • CD 195 antibody
      • CD195 antibody
      • CD195 Antigen antibody
      • Chemokine C C motif receptor 5 antibody
      • Chemokine receptor CCR5 antibody
      • CHEMR13 antibody
      • CKR 5 antibody
      • CKR5 antibody
      • CMKBR 5 antibody
      • CMKBR5 antibody
      • FLJ78003 antibody
      • HIV 1 Fusion Coreceptor antibody
      • HIV-1 fusion coreceptor antibody
      • HIV1 fusion coreceptor antibody
      • IDDM22 antibody
      • MIP-1 alpha receptor antibody
      see all

    画像

    • Naive T cells from healthy donor were Th1 derived. The gating strategy used was for alive cells. ab11466 was used at a 1/100 dilution.

      See Abreview

    • Flow Cytometry analysis of murine splenocytes using ab11466 at a 1/100 dilution.

      See Abreview

    • ab11466 staining CCR5 in murine spleen tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed with acetone, blocked using 5% goat serum for 1 hour at 25°C and then incubated with ab11466 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat polyclonal used at a 1/400 dilution.

      See Abreview

    参考文献

    ab11466 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Dog Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    投稿 Nov 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen from immune mouse)
    Specification
    Spleen from immune mouse
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Username

    Abcam user community

    Verified customer

    投稿 Apr 20 2012

    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (Whole blood)
    Specification
    Whole blood
    Preparation
    Cell harvesting/tissue preparation method: After red blood cell lysis
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Username

    Abcam user community

    Verified customer

    投稿 Apr 20 2012

    Thank you for your inquiry. Unfortunately, I cannot recommend any of these antibodies to your customer. The immunogen for these antibodies was the full-length CCR5 protein. We can suggest that all of them will recognize an epitope in the extracellula...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (splenocytes)
    Specification
    splenocytes
    Preparation
    Cell harvesting/tissue preparation method: red blood cells were lysed
    Sample buffer: PBS/BSA 1% Azide
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    投稿 Nov 18 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (Th1 cells)
    Specification
    Th1 cells
    Preparation
    Cell harvesting/tissue preparation method: Naive T cells from healthy donnor were Th1 derived
    Sample buffer: PBS/BSA 1%/Azide
    Permeabilization
    No
    Gating Strategy
    alive cells
    Username

    Abcam user community

    Verified customer

    投稿 Nov 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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