製品の概要

  • 製品名Anti-CCR3 antibody [Y31]
    CCR3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y31] to CCR3
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque Monkey
  • 免疫原

    A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CKR3.

  • ポジティブ・コントロール
    • K562 and Daudi cell lysates and human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-CCR3 antibody (HRP) [Y31] (ab200020)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
    • 組織特異性In eosinophils as well as trace amounts in neutrophils and monocytes.
    • 配列類似性Belongs to the G-protein coupled receptor 1 family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B chemokine receptor antibody
      • b-chemokine receptor antibody
      • C C chemokine receptor type 3 antibody
      • C C CKR 3 antibody
      • C-C chemokine receptor type 3 antibody
      • C-C CKR-3 antibody
      • CC chemokine receptor 3 antibody
      • CC chemokine receptor type 3 antibody
      • CC CKR 3 antibody
      • CC R3 antibody
      • CC-CKR-3 antibody
      • CCCKR3 antibody
      • CCR 3 antibody
      • CCR-3 antibody
      • CCR3 antibody
      • CCR3_HUMAN antibody
      • CD 193 antibody
      • CD193 antibody
      • CD193 antigen antibody
      • Chemokine (C C motif) receptor 3 antibody
      • chemokine (C-C motif) receptor 3 antibody
      • Chemokine (CC) receptor 1 like 2 antibody
      • Chemokine CC motif receptor 3 antibody
      • Chemokine receptor 3 antibody
      • CK R3 antibody
      • CKR 3 antibody
      • CKR3 antibody
      • CMKB R3 antibody
      • CMKBR 3 antibody
      • CMKBR1L2 antibody
      • CMKBR3 antibody
      • Eosinophil CC chemokine receptor 3 antibody
      • Eosinophil eotaxin receptor antibody
      • Eotaxin receptor antibody
      • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
      • MGC102841 antibody
      • MIP1 Alpha RL2 antibody
      see all

    Anti-CCR3 antibody [Y31] 画像

    • Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
    • All lanes : Anti-CCR3 antibody [Y31] (ab32512) at 1/500 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Daudi cell lysate


      Predicted band size : 43 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Anti-CCR3 antibody [Y31] (ab32512) 使用論文

    This product has been referenced in:
    • Sun RL  et al. CC-Chemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CC-Chemokine Receptor 2 (CCR2) Induction and p42/44 Mitogen-activated Protein Kinase (MAPK) Activation in Human Endothelial Cells. J Biol Chem 291:19532-44 (2016). WB ; Human . Read more (PubMed: 27458015) »
    • Orzekowsky-Schroeder R  et al. Probing the immune and healing response of murine intestinal mucosa by time-lapse 2-photon microscopy of laser-induced lesions with real-time dosimetry. Biomed Opt Express 5:3521-40 (2014). Read more (PubMed: 25360369) »

    See all 8 Publications for this product

    Product Wall

    Thank you very much for your reply and for sending this additional information.

    Your credit note ID is ***.

    I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the origina...

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    Thank you very much for contacting us and letting us know about the trouble with these antibodies.

    Thank you also for sending so many details of your protocol.The protocol seems to be ok and similar to the protocol that the lab uses to test t...

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    Thank you for your inquiry.

    In general we recommend to use EDTA pH9. This antigen retrieval method is optimal for the most antigens. Please see the attachment and this publication (Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Jurkat cells)
    Loading amount 100000 cells
    Specification Jurkat cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"