製品の概要

  • 製品名Anti-CCNO antibody
    CCNO 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CCNO
  • 特異性At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 14 amino acid peptide from near the amino terminus of human Cyclin O(Genbank accession No. NP_066970).

  • ポジティブ・コントロール
    • Mouse bladder tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-CCNO antibody 画像

  • ICC/IF image of ab47682 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47682, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab47682 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47682, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 38 kDa


    Western blot analysis of ab47682 in mouse bladder tissue lysate with ab47682 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.

Anti-CCNO antibody (ab47682) 使用論文

This product has been referenced in:
  • Orthwein A  et al. Regulation of activation-induced deaminase stability and antibody gene diversification by Hsp90. J Exp Med 207:2751-65 (2010). WB ; Human . Read more (PubMed: 21041454) »

See 1 Publication for this product

Product Wall

The exact sequence is proprietary, but the immunogen overlaps the amino terminus of the region specified, starting a little before it.


I hope this is helpful. Please let us know if you have any more questions.

Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement with the order number 1062123.

To check the status of the order please contact our Customer Service team and...

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Please do not send the vial back to us. The used vial is no use to us.

I am sending you a vial Anti-DNA Polymerase beta antibody (ab26343) today. Please double check the target name and confirm that this the right antibody you want.
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Thank you for contacting us. I apologize for the confusion which happened due to confusion in literature.

My colleague from datasheet department has confirmed that they never changed the target name for this product it was always CCNO. So cou...

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Thank you for contacting us.

After conducting further research about he target I have to say the antibody is specific to CCNO. Swissprot ID P22674.

The UNG2 and CCNO target were used as similar in past. However this antibody is agia...

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Thank you for contacting us.

I can confirm that the antibody is anti UNG2. Please use the antibody without any hesitation.

I have also requested the datasheet team to change the target name which will be corrected soon.

...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E8.5 mouse embryo sections)
Specification E8.5 mouse embryo sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step Novocastra protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Feb 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"