製品の概要

  • 製品名Anti-CCNL1 antibody
    CCNL1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CCNL1
  • アプリケーション適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human CCNL1 protein (AAH38394, 1 a.a. ~ 173 a.a), with a tag.

  • ポジティブ・コントロール
    • CCNL1 transfected 293T cell lysate. Hela cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
ELISA
  • 追加情報ELISA: Use at an assay dependent concentration. Detection limit for recombinant tagged CCNL1 is approximately 3ng/ml as a capture antibody.
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: Isoform 5 (Cyclin L gamma) 18kDa; Longest Isoform (Cyclin L alpha) 60 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcriptional regulator which participates in regulating the pre-mRNA splicing process. Seems to be involved in the regulation of RNA polymerase II (pol II). Functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. May be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). Inhibited by the CDK-specific inhibitor p21.
    • 組織特異性Ubiquitous with higher level in thymus. Overexpression in primary tumors of head and neck squamous cell carcinomas (HNSCC).
    • 配列類似性Belongs to the cyclin family. Cyclin L subfamily.
    • ドメインContains a RS region (arginine-serine dipeptide repeat) within the C-terminal domain which is the hallmark of the SR family of splicing factors. This region probably plays a role in protein-protein interactions.
    • 細胞内局在Nucleus speckle. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ania 6a antibody
      • CCNL1 antibody
      • CCNL1_HUMAN antibody
      • Cyclin L antibody
      • Cyclin L gamma antibody
      • Cyclin L1 antibody
      • Cyclin-L antibody
      • Cyclin-L1 antibody
      see all

    Anti-CCNL1 antibody 画像

    • All lanes : Anti-CCNL1 antibody (ab68353) at 1 µg/ml

      Lane 1 : CCNL1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescent staining of CCNL1 in HeLa cells using ab68353 at 10µg/ml.

    Anti-CCNL1 antibody (ab68353) 使用論文

    ab68353 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab68353.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"