製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CCN1
  • アプリケーション
    適用あり: ICC/IF, IHC-FoFr, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse CCN1. Synthetic peptide surrounding amino acid 166 of mouse Cyr61. The peptide was 10-15 amino acids long.
    (Peptide available as ab52125)

  • ポジティブ・コントロール
    • Jurkat cell lysate, mouse small intestine lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50. PubMed: 19077055
IHC-FoFr Use at an assay dependent concentration. PMID 17724216
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21490242
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42 kDa).Can be blocked with CCN1 peptide (ab52125).

ターゲット情報

  • 機能
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • 配列類似性
    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3CH61 antibody
    • CCN 1 antibody
    • CCN family member 1 antibody
    • CYR 61 antibody
    • Cyr61 antibody
    • CYR61 protein antibody
    • CYR61_HUMAN antibody
    • Cysteine rich angiogenic inducer 61 antibody
    • Cysteine rich heparin binding protein 61 antibody
    • Cysteine-rich angiogenic inducer 61 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • IBP-10 antibody
    • IGF-binding protein 10 antibody
    • Igfbp 10 antibody
    • IGFBP-10 antibody
    • Igfbp10 antibody
    • Insulin like growth factor binding protein 10 antibody
    • Insulin-like growth factor-binding protein 10 antibody
    • Protein CYR61 antibody
    • Protein GIG1 antibody
    see all

Anti-CCN1 antibody 画像

  • All lanes : Anti-CCN1 antibody (ab24448) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse small intestine tissue lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ICC/IF image of ab24448 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma cells labeling CCN1 with ab24448 at 1/100 dilution. Cells were fixed with paraformaldehyde and blocked with 0.05% triton-X100. The cells were blocked with 3% BSA for 1 hour at 20°C followed by incubation with ab24448 in TBS/Tween-20 + 1% BSA for 2 hours at 20°C. A Donkey anti-rabbit Cy3® secondary antibody was used at 1/200 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of human synovial fibroblasts labeling CCN1 with ab24448 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with methanol. The cells were blocked with 1% BSA for 1 hour at 25°C. The cells were incubated with PBS with ab24448 in 1% BSA for 2 hours at 25°C. A goat anti-rabbit Cy5® secondary antibody was used.

    See Abreview

  • ab24448 staining Human myometrium. Staining can be observed in the cytoplasm and nuclear compartments.
    Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-CCN1 antibody (ab24448) 使用論文

This product has been referenced in:
  • Diamantopoulou Z  et al. TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells. Cancer Cell 31:621-634.e6 (2017). Mouse . Read more (PubMed: 28416184) »
  • Zhang D  et al. Fibulin-4 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition via the PI3K/Akt/mTOR pathway. Int J Oncol 50:1513-1530 (2017). IHC-P, ICC/IF ; Human . Read more (PubMed: 28339091) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse Knee:- Tibial Plateau)
Specification
Mouse Knee:- Tibial Plateau
Permeabilization
No
Fixative
10% neutral buffered formalin for 48 hours then decalcified in 14% EDTA pH 7.4 for 7 days
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Abcam user community

Verified customer

投稿 Jun 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (osteosarcoma)
Specification
osteosarcoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer - 70°C - ON
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 30 2012

Thank you for contacting us.
This antibody has not yet been tested in ELISA, therefore, we do not know for sure if and how well it might work in this application.
It works well in other applications (IHC, ICC), thus it might work well in ELIS...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (synovial fibroblasts)
Specification
synovial fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - methanol, 15min -20 degrees
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Torsten Lowin

Verified customer

投稿 Mar 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (osteoblast)
Loading amount
40 µg
Specification
osteoblast
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
blocking buffer Sigma as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (osteosarcoma)
Specification
osteosarcoma
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% triton-X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (glioma)
Loading amount
80 µg
Specification
glioma
Gel Running Conditions
Reduced Denaturing
Blocking step
odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (osteosarcoma (bone tumor))
Loading amount
15 µg
Specification
osteosarcoma (bone tumor)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5
Username

Abcam user community

Verified customer

投稿 Oct 10 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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