製品の概要

  • 製品名Anti-CCN1 antibody
    CCN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CCN1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human CCN1.

    (Peptide available as ab24416.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Hela nuclear cell extract Hela cell lysate. A431 cell lysate. Jurkat cell lysate Liver (Mouse) tissue lysate Heart (Mouse) tissue lysate Kidney (Mouse) tissue lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab10760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報IHC-P: Use at a dilution of 1/100 for 1 hr.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 42-50 kDa (predicted molecular weight: 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
    • 配列類似性Belongs to the CCN family.
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 1 IGFBP N-terminal domain.
      Contains 1 TSP type-1 domain.
      Contains 1 VWFC domain.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 3CH61 antibody
      • CCN 1 antibody
      • CCN family member 1 antibody
      • CYR 61 antibody
      • Cyr61 antibody
      • CYR61 protein antibody
      • CYR61_HUMAN antibody
      • Cysteine rich angiogenic inducer 61 antibody
      • Cysteine rich heparin binding protein 61 antibody
      • Cysteine-rich angiogenic inducer 61 antibody
      • GIG 1 antibody
      • GIG1 antibody
      • IBP-10 antibody
      • IGF-binding protein 10 antibody
      • Igfbp 10 antibody
      • IGFBP-10 antibody
      • Igfbp10 antibody
      • Insulin like growth factor binding protein 10 antibody
      • Insulin-like growth factor-binding protein 10 antibody
      • Protein CYR61 antibody
      • Protein GIG1 antibody
      see all

    Anti-CCN1 antibody 画像

    • ab10760 staining CCN1 in mouse kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in Tris-EDTA buffer pH9.0 and bloking for 15 minutes at 20°C (5 minutes/peroxidase block and 10 minutes/protein block). The primary antibody was diluted 1/100 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used, undiluted as the secondary.  

      See Abreview

    • All lanes : Anti-CCN1 antibody (ab10760) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Heart (Mouse) Tissue Lysate
      Lane 3 : Kidney (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
      Additional bands at : 35 kDa,85 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-CCN1 antibody (ab10760) at 1/500 dilution

      Lane 1 : Hela nuclear cell extract
      Lane 2 : Hela whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : Jurkat whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution

      Predicted band size : 42 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Anti-CCN1 antibody (ab10760) 使用論文

    ab10760 has not yet been referenced specifically in any publications.

    Product Wall



    Our products are not intended for in vivo use, so unfortunately we don't have any recommendations for this kind of experiment. This antibody has only been tested in Western blot and immunohistochemistry, so we are also not sure if it will suc...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA pH9.0
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Oct 30 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Oct 30 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"