Anti-CCN1 抗体 [3H3] (ab80112)


  • 製品名Anti-CCN1 antibody [3H3]
    CCN1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3H3] to CCN1
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Recombinant fragment: IKDPMEDQDG LLGKELGFDA SEVELTRNNE LIAVGKGSSL KRLPVFGMEP RILYNPLQGQ KCIVQTTSWS QCSKT, corresponding to amino acids 168-242 of human CCN1 purified from E. coli

  • ポジティブ・コントロール
    • Jurkat cell lysates.
  • 特記事項

    ab80112 is derived from hybridization of mouse FO myeloma cells with spleen cells from immunized BALB/c mice.
    The isotype is mouse IgG1 heavy chain and kappa light chain.




Our Abpromise guarantee covers the use of ab80112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/50.
WB 1/250 - 1/1000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.


  • 機能Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • 配列類似性Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3CH61 antibody
    • CCN 1 antibody
    • CCN family member 1 antibody
    • CYR 61 antibody
    • Cyr61 antibody
    • CYR61 protein antibody
    • CYR61_HUMAN antibody
    • Cysteine rich angiogenic inducer 61 antibody
    • Cysteine rich heparin binding protein 61 antibody
    • Cysteine-rich angiogenic inducer 61 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • IBP-10 antibody
    • IGF-binding protein 10 antibody
    • Igfbp 10 antibody
    • IGFBP-10 antibody
    • Igfbp10 antibody
    • Insulin like growth factor binding protein 10 antibody
    • Insulin-like growth factor-binding protein 10 antibody
    • Protein CYR61 antibody
    • Protein GIG1 antibody
    see all

Anti-CCN1 antibody [3H3] 画像

  • Anti-CCN1 antibody [3H3] (ab80112) at 1/250 dilution + Jurkat cell lysate at 40 µg

    goat anti-mouse HRP
    developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab80112 at 1/50 dilution staining CCN1 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) staining technique.

  • ICC/IF image of ab80112 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80112, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CCN1 antibody [3H3] (ab80112) 使用論文

ab80112 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Cell lysate - whole cell (osteosarcoma)
Specification osteosarcoma
Total protein in input 200 µg

Abcam user community

Verified customer

投稿 Jul 15 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (osteosarcoma)
Specification osteosarcoma
Total protein in input 200 µg

Abcam user community

Verified customer

投稿 Jun 26 2014

Thank you for contacting us.

I confirm the sodium citrate concentration used was 0.1M.

Both 0.1M and 0.01M are fine. In our online protocols we specify using 0.01M concentration for the buffer, but if 0.1M concentration is used inst...

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