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Our Abpromise guarantee covers the use of ab62074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 118 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
ab62074 staining CCK4 in E9.5 mouse embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 5% donkey serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Image shows sagittal section of E9.5 mouse embryo. Neuroectoderm and underlying mesoderm labelled with ab62074 in green. DAPI stained nuclei in blue.
ab62074 at 5 µg/ml staining CCK4 in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
ab62074 has not yet been referenced specifically in any publications.