Anti-CCDC80 抗体 (ab75881)

製品の概要

  • 製品名Anti-CCDC80 antibody
  • 製品の詳細
    Rabbit polyclonal to CCDC80
  • アプリケーション適用あり: ICC/IF, WB, ICC, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from C terminal domain of human CCD80

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB 1/200 - 1/2000. Predicted molecular weight: 108 kDa.
ICC Use at an assay dependent concentration.
IHC-P 1/50 - 1/500.

ターゲット情報

  • 機能Promotes cell adhesion and matrix assembly.
  • 組織特異性Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.
  • 配列類似性Belongs to the CCDC80 family.
  • 翻訳後修飾Phosphorylated.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610001E17Rik antibody
    • CCD50 antibody
    • CCD80_HUMAN antibody
    • CCDC80 antibody
    • CL2 antibody
    • Coiled coil domain containing 80 antibody
    • Coiled coil domain containing protein 50 antibody
    • Coiled-coil domain-containing protein 80 antibody
    • Down regulated by oncogenes protein 1 antibody
    • Down-regulated by oncogenes protein 1 antibody
    • DRO1 antibody
    • MGC131805 antibody
    • MGC134851 antibody
    • Nuclear envelope protein okuribin antibody
    • okuribin antibody
    • OTTMUSP00000030743 antibody
    • Protein Ymer antibody
    • SSG1 antibody
    • steroid sensitive gene 1 antibody
    • steroid sensitive protein 1 antibody
    • Up-regulated in BRS-3 deficient mouse homolog antibody
    • URB antibody
    see all

Anti-CCDC80 antibody 画像

  • ICC/IF image of ab75881 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75881, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CCDC80 antibody (ab75881) 使用論文

ab75881 has not yet been referenced specifically in any publications.

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Wenn Sie mir allerdings eine Liste mit den Firmennamen zukommen lassen, deren Antikörper Sie bereits verwendet haben, k...

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Vielen Dank für Ihren Anruf.

Ich habe im Labor nachgefragt, und es sieht so aus, als ob uns gleich zwei Fehler unterlaufen sind: Natürlich muss der Antikörper Anti-CCDC80 und nicht Anti-CCD80 heißen. Desweiteren ist der ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (lung epithelial cells)
Specification lung epithelial cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton x-100 0.2%
Blocking step PBS 3%BSA, 0.4% Tritonx-100 as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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投稿 May 16 2012

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I am very pleased to hear you would like to accept our offer and test ab75881 in mouse tissues. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code ...

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Thank you very much for your interest in ab75881.

Ab75881 has been predicted to work in Mouse and Rat as the protein sequences of those species contains over 85% homology with the synthetic peptide derived from C terminal domain of human CCD80...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"