Anti-CCDC75 抗体 (ab122451)
Key features and details
- Rabbit polyclonal to CCDC75
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CCDC75 antibody -
製品の詳細
Rabbit polyclonal to CCDC75 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: KSGIGHEASL KRKAEEKLES YRKKIHMKNQ AEEKAAEQFR MRLKNKQDEM KLEGDLRRSQ RACQQLDVQK NIQVPREAWY WL, corresponding to amino acids 107-188 of Human CCDC75
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ポジティブ・コントロール
- Human testis tissue; RT4, U251 MG, Human Liver and Tonsil lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122451の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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配列類似性
Contains 1 G-patch domain. - Information by UniProt
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参照データベース
- Entrez Gene: 253635 Human
- SwissProt: Q8N954 Human
- Unigene: 595250 Human
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別名
- CCD75_HUMAN antibody
- CCDC75 antibody
- CENP Y antibody
see all
画像
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab122451 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122451, at 1/200, staining CCDC75 in paraffin embedded Human testis tissue by immunohistochemistry.
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All lanes : Anti-CCDC75 antibody (ab122451) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122451 は論文での使用が確認できていません。